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SOUTHERN BLOT [1 record]
Record 1 - internal organization data 2013-07-15
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- Southern blot
1, record 1, English, Southern%20blot
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- Southern blotting 2, record 1, English, Southern%20blotting
correct
- Southern blot method 3, record 1, English, Southern%20blot%20method
correct, less frequent
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A method used in gene cloning to separate and identify specific DNA fragments. 4, record 1, English, - Southern%20blot
Record number: 1, Textual support number: 1 OBS
After separation by agarose gel electrophoresis, a nitrocellulose film is placed on the gel surfaces and dry blotting material on the nitrocellulose. Liquid is thus transported from a reservoir beneath the gel through the gel and nitrocellulose filter. The film adsorbs DNA fragments which are then visualized using a complementary DNA or RNA radioactive probe. 4, record 1, English, - Southern%20blot
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- transfert de Southern
1, record 1, French, transfert%20de%20Southern
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- transfert de type Southern 2, record 1, French, transfert%20de%20type%20Southern
correct, masculine noun
- Southern blot 3, record 1, French, Southern%20blot
avoid, see observation, masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Méthode de clonage des gènes qui consiste à transférer des fragments d'ADN préalablement dénaturés sur filtre de nitrocellulose depuis un gel d'agarose. 4, record 1, French, - transfert%20de%20Southern
Record number: 1, Textual support number: 1 OBS
Il y a alors hybridation du filtre avec une (ou plusieurs) sonde(s), et révélation des fragments hybridés par radioautographie. 4, record 1, French, - transfert%20de%20Southern
Record number: 1, Textual support number: 2 OBS
Bien que le terme «Southern blot» soit en usage dans la communauté scientifique francophone, il est préférable d'utiliser le terme français «transfert de Southern» ou «transfert Southern». 5, record 1, French, - transfert%20de%20Southern
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
Record 1, Main entry term, Spanish
- técnica de Southern blot
1, record 1, Spanish, t%C3%A9cnica%20de%20Southern%20blot
feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
- transferencia de mancha Southern 1, record 1, Spanish, transferencia%20de%20mancha%20Southern
feminine noun
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 CONT
La técnica de Southern blot permite el análisis de ADN genómico o fragmentos definidos de ADN después de digestión con endonucleasas de restricción. 1, record 1, Spanish, - t%C3%A9cnica%20de%20Southern%20blot
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