TERMIUM Plus®

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enzyme-linked immunospot technique [1 record]

Record 1 2006-05-08

English

Subject field(s)
  • Biochemistry
  • Immunology
CONT

ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for a protein followed by an appropriate incubation period. Subsequently, the protein of interest is detected in the environment immediately surrounding the cell secreting it, with another antibody specific for a different epitope of the protein.

French

Domaine(s)
  • Biochimie
  • Immunologie
CONT

Des nouvelles méthodes d'exploration de la réponse immune, comme l'Elispot (enzyme-linked immunospot) et l'utilisation par cytométrie en flux de molécules HLA [human leucocyte antigen] solubles, permettent de détecter et de compter les cellules productrices de telle ou telle cytokine. Elles conduisent à des analyses immunologiques très rapides sur des malades volontaires, et à une bien meilleure compréhension de l'histoire naturelle de la maladie.

Spanish

Campo(s) temático(s)
  • Bioquímica
  • Inmunología
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