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CARTE CLONES CHEVAUCHANTS [1 record]
Record 1 - internal organization data 2011-10-28
Record 1, English
Record 1, Subject field(s)
- Biotechnology
Record 1, Main entry term, English
- contig map
1, record 1, English, contig%20map
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- cosmid contig map 2, record 1, English, cosmid%20contig%20map
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A map depicting the relative order of a linked library of small overlapping clones representing a complete chromosomal segment. 3, record 1, English, - contig%20map
Record number: 1, Textual support number: 1 OBS
Contig maps: Bottom-up mapping. The bottom-up approach involves cutting the chromosomes into small pieces, each of which is cloned and ordered. The ordered fragments form contiguous DNA blocks (contigs). Currently, the resulting library of clones varies in size from 10,000 bp to 1 Mb. An advantage of this approach is the accessibility of these stable clones to other reseachers. Contig construction can be verified by FISH, which localizes cosmids to specific regions within chromosomal bands. 2, record 1, English, - contig%20map
Record 1, French
Record 1, Domaine(s)
- Biotechnologie
Record 1, Main entry term, French
- carte des fragments chevauchants
1, record 1, French, carte%20des%20fragments%20chevauchants
proposal, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- carte des clones chevauchants 1, record 1, French, carte%20des%20clones%20chevauchants
proposal, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La stratégie des contigs consiste à découper le DNA génomique en fragments chevauchants d'environ 50 kb qui sont clonés dans un cosmide. La carte de restriction de chaque clone individuel est étudiée et introduite dans un ordinateur, qui effectue la comparaison et étudie les clones ayant des fragments communs. À l'aide des premiers clones contigus, d'où le nom de «contigs», on peut progressivement ordonner les clones. Cette stratégie s'est révélée très fructueuse pour dresser une première carte des 80 mégabases du génome de Caenorhabditis elegans. 2, record 1, French, - carte%20des%20fragments%20chevauchants
Record 1, Spanish
Record 1, Textual support, Spanish
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