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AFFINITY PRECIPITATION [1 record]
Record 1 - internal organization data 2010-05-19
Record 1, English
Record 1, Subject field(s)
- Biological Sciences
Record 1, Main entry term, English
- affinity precipitation
1, record 1, English, affinity%20precipitation
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
Purification technique in which the ligand is attached to a soluble carrier, either forming bifunctional reagents capable of directly precipitating oligomeric proteins, or giving polyfunctional polymer derivatives which can be precipitated by, for example a change of pH. 2, record 1, English, - affinity%20precipitation
Record number: 1, Textual support number: 1 CONT
Two affinity precipitation approaches ... have been published. In one of these the affinity ligand is derivatized to form bifunctional reagents, also called bis-ligands, which react with oligomeric proteins.... The other approach, however, is of more general applicability. Here, the affinity ligands are attached to polymers forming multifunctional soluble reagents which, after complex formation with the product of interest, are caused to aggregate and finally precipitate by changing the composition or properties of solution, e.g. by the addition of salt or by changing the pH. 2, record 1, English, - affinity%20precipitation
Record 1, French
Record 1, Domaine(s)
- Sciences biologiques
Record 1, Main entry term, French
- précipitation par affinité
1, record 1, French, pr%C3%A9cipitation%20par%20affinit%C3%A9
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Purification par affinité qui consiste à faire précipiter le complexe ligand/molécule ou macroligand/molécule à purifier. Les premiers travaux ont tiré profit d'agents bifonctionnels et affines pour la molécule à purifier, tel le BIS-NAD (N2, N2-adipohydrazido-bis (N6-carbonylméthyl)-NAD). En présence de pyruvate, cet agent, affine pour la lactate déshydrogénase (LDH), précipite l'enzyme sous forme d'agrégats macromoléculaires. Après récupération des agrégats par centrifugation, l'enzyme est dissociée du BIS-NAD par addition de NADH. 2, record 1, French, - pr%C3%A9cipitation%20par%20affinit%C3%A9
Record 1, Spanish
Record 1, Textual support, Spanish
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