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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

A procedure which uses a gel matrix combined with an enzyme or nucleic acid to obtain purification of biomolecules.  3, record 1, English, - affinity%20chromatography

Record number: 1, Textual support number: 2 DEF

A technique of molecular separation in which molecules are attached to an insoluble (e.g., sepharose) matrix. Only those molecules which show affinity to the bound molecule (e.g., an antibody for its antigen) are retained. These trapped molecules can be subsequently eluted.  4, record 1, English, - affinity%20chromatography

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Technique permettant la séparation de substances, [...] qui utilise la fixation spécifique d'une protéine sur un ligand lui-même fixé par liaison covalente à un support microscopique.  3, record 1, French, - chromatographie%20d%27affinit%C3%A9

Record number: 1, Textual support number: 1 CONT

[...] la chromatographie d'affinité permet de purifier une protéine enzymatique par exemple. La méthode consiste à attacher de façon covalente sur un support solide un analogue stérique du substrat de l'enzyme (inhibiteur compétitif ou substrat modifié) en vue de former avec l'enzyme un complexe du type enzyme-substrat [...]. L'enzyme sera donc retardé lors du passage sur la colonne par rapport aux autres protéines qui, ne reconnaissant pas l'analogue, sortiront de la colonne plus rapidement que l'enzyme.  4, record 1, French, - chromatographie%20d%27affinit%C3%A9

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

Purification technique based on a selective and reversible formation of a complex between the substance to be purified and another substance, most often called the ligand.  2, record 2, English, - affinity%20purification

Record number: 2, Textual support number: 1 CONT

Although affinity chromatography is still the dominant affinity purification technique, others - such as batch affinity adsorption, affinity precipitation and affinity partitioning - offer certain advantages in large-scale work.... Enzymes normally display highly specific interactions with their substrates. Advantage can be taken of this specificity in the so-called affinity separation methods of enzyme purification, i.e. affinity chromatography and affinity elution.  3, record 2, English, - affinity%20purification

Record number: 2, Textual support number: 1 OBS

Depending on the way in which the ligand is derivatized and physically contained, affinity purification methods are classified as belonging to one of the following three categories: affinity adsorption, affinity precipitation and affinity partitioning.  3, record 2, English, - affinity%20purification

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

De nombreux travaux tentent d'améliorer la productivité de la purification par affinité en l'adaptant (...) à des procédés de séparation capables de traiter des grands volumes en un minimum de temps. Il s'agit de la filtration par affinité à contre-courant, de la séparation d'affinité par partage de phase et de la précipitation par affinité.  1, record 2, French, - purification%20par%20affinit%C3%A9

Record 2,  Textual support,  Spanish