TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

BRIGHT FIELD OPTICAL MICROSCOPY [1 record]

Record 1 2015-09-09

English

Subject field(s)
  • Optics
  • Biophysics
  • Chemistry
CONT

Brightfield microscopy is the most elementary form of microscope illumination techniques ... The name "brightfield" is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field. ... In brightfield microscopy a specimen is placed on the stage of the microscope and incandescent light from the microscope’s light source is aimed at a lens beneath the specimen. ... light passes through the specimen and then is collected by an objective lens situated in a turret above the stage. ... This technique can be used to view fixed specimens or live cells. Since many organic specimens are transparent or opaque, staining is required to cause the contrast that allows them to be visible under the microscope.

Key term(s)
  • brightfield optical microscopy
  • brightfield microscopy

French

Domaine(s)
  • Optique
  • Biophysique
  • Chimie
CONT

La microscopie optique en champ clair (ou «à fond clair») est la plus simple et la plus ancienne des techniques de microscopie. [...] L'illumination se fait par transmission de lumière blanche, c'est-à-dire que l'échantillon est illuminé par dessous et observé par dessus. Les limitations de cette technique sont principalement un faible contraste de la plupart des échantillons biologiques et une résolution faible due au flou créé par la matière hors du plan focal. En contrepartie, la technique est simple et l'échantillon ne nécessite qu'une préparation minime.

OBS

La microscopie à fond clair permet d'observer des préparations colorées (sans coloration, on ne voit rien).

Spanish

Save record 1

Copyright notice for the TERMIUM Plus® data bank

© Public Services and Procurement Canada, 2024
TERMIUM Plus®, the Government of Canada's terminology and linguistic data bank
A product of the Translation Bureau

Features

Language Portal of Canada

Access a collection of Canadian resources on all aspects of English and French, including quizzes.

Writing tools

The Language Portal’s writing tools have a new look! Easy to consult, they give you access to a wealth of information that will help you write better in English and French.

Glossaries and vocabularies

Access Translation Bureau glossaries and vocabularies.

Date Modified: