TERMIUM Plus®

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Record 1 2003-05-09

English

Subject field(s)
  • Molecular Biology
  • Genetics
CONT

Frozen cells (about 20 g wet weight) harvested from about 150 liter culture with sodium thiosulfate were suspended in 270 ml of 10 mM Tris-HCl buffer, pH 8.0, and then disrupted in a French pressure cell (1,100 kg/cm2). All subsequent steps were performed under aerobic conditions at room temperature. Unbroken cells were removed by centrifugation at 10,000 x g for 15 min. The resulting supernatant was used as a cell-free extract.

French

Domaine(s)
  • Biologie moléculaire
  • Génétique
DEF

Broyat cellulaire plus ou moins purifié capable d'assurer certaines fonctions enzymatiques du vivant in vitro.

Spanish

Campo(s) temático(s)
  • Biología molecular
  • Genética
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