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CYANOGEN BROMIDE METHOD [1 record]

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Record 1 1990-01-16

English

Subject field(s)
  • Biological Sciences
DEF

Approach used for the laboratory preparation of immobilized enzyme derivatives as well as for insoluble adsorbents for affinity chromatography. It is based on the activation of water-insoluble polysaccharides, cellulose, cross-linked dextran, and agarose, with cyanogen bromide.... The polysaccharide is activated with cyanogen bromide at high pH (10-11.5). Compounds containing free amino groups, including peptides and proteins, can then be attached covalently to the activated polymer at mildly alkaline pH values.

French

Domaine(s)
  • Sciences biologiques

Spanish

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