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DENATURING GRADIENT GEL ELECTROPHORESIS [1 record]

Record 1 2013-07-18

English

Subject field(s)
  • Biochemistry
CONT

High-resolution HLA-DRB [human leucocyte antigen-5,6-dichloro-furanosyl-benzimidazole riboside] typing is required for bone marrow transplantation between unrelated donors and recipients and also for identification of novel HLA-DRB alleles. Here we describe a method for the unambiguous identification of HLA-DRB alleles using the polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct sequencing.

French

Domaine(s)
  • Biochimie

Spanish

Campo(s) temático(s)
  • Bioquímica
DEF

Método de electroforesis para separar fragmentos de ADN del mismo tamaño en función de su secuencia.

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