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DNA-RNA HYBRIDIZATION [1 record]

Record 1 2011-07-11

English

Subject field(s)
  • Genetics
DEF

A process based on the fact that isolated RNA on being mixed with denatured (single-stranded) DNA under appropriate conditions forms hydrogen bonds with the complementary DNA sequences leading to DNA-RNA hybrids.

OBS

The rate of hybridization is limited by the collision frequency of complementary nucleotide sequences. It is a useful tool for localizing genes in chromosomes.

French

Domaine(s)
  • Génétique
DEF

Formation in vitro d'hybrides ADN-ARN par appariement des séquences nucléotidiques complémentaires appartenant à un même brin ou deux brins différents.

Spanish

Campo(s) temático(s)
  • Genética
CONT

Las técnicas de desnaturalización y posterior renaturalización se utilizan para conseguir la hibridación de ácidos nucleicos, es decir, una vez separadas las dos hebras del ADN doble hélice, es posible volver a formar una doble hélice con una hebra de ADN que sea complementaria (hibridación de ADN con ADN) o volver a formar una doble hélice con un segmento de ARN que contenga la secuencia complementaria (hibridación de ADN con ARN).

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