TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

ENZYME EXTRACTION [2 records]

Record 1 2011-08-01

English

Subject field(s)
  • Medical and Surgical Equipment
  • Bioengineering
  • Blood
OBS

Term(s) taken from a Canadian laboratory-equipment company's catalogue.

French

Domaine(s)
  • Équipement médico-chirurgical
  • Technique biologique
  • Sang
OBS

Terme(s) tiré(s) du catalogue d'une compagnie canadienne d'équipement de laboratoire.

Spanish

Save record 1

Record 2 2003-05-09

English

Subject field(s)
  • Biological Sciences
  • Biotechnology
DEF

The removal of enzymes from contaminating materials in order to increase their specific activity. Techniques fall into two groups: those used to separate enzymes from solid substrate culture; those used to release enzymes from the interior of microbial cells. Where enzymes produced in solid fermentation are of the extracellular type, they may be removed by countercurrent washing. If enzymes are intracellular, the cells must be broken using chemical or biochemical methods.

CONT

Enzyme extraction/cell disruption [methods include:] Physical methods: (a) Solid shear, (b) agitation with abrasives, (c) liquid shear, (d) freeze-thawing. Chemical methods: (e) detergents, (f) enzyme treatment, (g) osmotic shock, (h) alkali treatment.

French

Domaine(s)
  • Sciences biologiques
  • Biotechnologie
CONT

L'extraction d'une enzyme contenue dans un tissu ou dans des cellules bactériennes commence par la destruction de l'organisation cellulaire par broyage, ultrasons, lyse des parois, par exemple. Les débris cellulaires sont séparés par centrifugation; le matériel soluble est recueilli et dialysé pour éliminer les petites molécules. Diverses méthodes sont ensuite utilisées.

Spanish

Campo(s) temático(s)
  • Ciencias biológicas
  • Biotecnología
Save record 2

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