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ISOELECTROFOCUSING [1 record]
Record 1 - internal organization data 2013-11-15
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- isoelectric focusing
1, record 1, English, isoelectric%20focusing
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- isoelectrofocusing 2, record 1, English, isoelectrofocusing
correct
- electrofocusing 2, record 1, English, electrofocusing
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
An analytical or separation procedure similar to gel electrophoresis. It is used to separate proteins and other charged molecules on the basis of their isoelectric point. A series of ampholytes are used to establish a pH gradient within a support gel. The charged molecules migrate through the gel until they reach an equilibrium point at which they carry no net charge and thus are not affected by the electric field across the gel. 1, record 1, English, - isoelectric%20focusing
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- électrofocalisation
1, record 1, French, %C3%A9lectrofocalisation
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- focalisation isoélectrique 2, record 1, French, focalisation%20iso%C3%A9lectrique
correct, feminine noun
- isoélectrofocalisation 2, record 1, French, iso%C3%A9lectrofocalisation
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Méthode de séparation très résolutive des protéines qui repose sur la distinction de ces macromolécules selon leur point isoélectrique (pHi). Chaque protéine en effet, comme n'importe quel ampholyte, est définie par son point isoélectrique, valeur du pH du milieu pour lequel sa charge électrique résultante est nulle. Placée à un pH supérieur à son pHi la protéine se charge négativement par perte de protons. A un pH inférieur au pHi, la protéine se charge positivement par capture de protons. Sous l'influence d'un champ électrique continu, les protéines migrent vers la zone de pH "en équilibre" avec leur pH isoélectrique, c'est-à-dire dans la zone où elles seront électriquement neutres. 2, record 1, French, - %C3%A9lectrofocalisation
Record 1, Spanish
Record 1, Textual support, Spanish
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