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PLUS-AND-MINUS METHOD [1 record]
Record 1 - internal organization data 2015-04-08
Record 1, English
Record 1, Subject field(s)
- Molecular Biology
Record 1, Main entry term, English
- plus-and-minus method
1, record 1, English, plus%2Dand%2Dminus%20method
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
... new [method] devised for sequencing DNA ... 1, record 1, English, - plus%2Dand%2Dminus%20method
Record number: 1, Textual support number: 1 CONT
In broad outline the plus-and-minus method calls for synthesizing pieces of DNA that are complementary to various regions of the DNA being sequenced. Because of the way the synthesis is manipulated each piece ends either with or just before a particular, identifiable nucleotide (A, G, T or C). By performing the synthesis to produce a large number of pieces, each one ending with a successive nucleotide in the sequence, it is possible to establish the sequence of the entire DNA molecule. 1, record 1, English, - plus%2Dand%2Dminus%20method
Record 1, French
Record 1, Domaine(s)
- Biologie moléculaire
Record 1, Main entry term, French
- méthode du plus-moins
1, record 1, French, m%C3%A9thode%20du%20plus%2Dmoins
feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- technique plus-moins 2, record 1, French, technique%20plus%2Dmoins
feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
[Nnouvelle méthode] pour établir la séquence de l'ADN [...] 1, record 1, French, - m%C3%A9thode%20du%20plus%2Dmoins
Record number: 1, Textual support number: 1 CONT
Dans ses grandes lignes, la méthode du «plus-moins» consiste en la synthèse de fragments d'ADN, complémentaires des zones de l'ADN dont on veut établir la séquence. La synthèse est orientée de telle façon que chaque fragment se termine par un nucléotide particulier que l'on peut identifier (A, G, T ou C) ou par le nucléotide qui le précède. En faisant la synthèse d'un grand nombre de fragments dont chacun se termine par un des nucléotides successifs de la séquence, il est possible d'établir la séquence entière de la molécule d'ADN. 1, record 1, French, - m%C3%A9thode%20du%20plus%2Dmoins
Record 1, Spanish
Record 1, Textual support, Spanish
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