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PRINS [1 record]

Record 1 1997-05-07

English

Subject field(s)
  • Genetics
CONT

Primed in situ labeling (PRINS) is a fast alternative to traditional in situ hybridization, requiring only a tew handling steps (1,2). In the PRINS reaction, an unlabeled sequence-specific oligonucleotide primer or short DNA fragment is hybridized (annealed) to denatures chromosomal DNA and extended in situ in the presence of hapten-labeled nucleotide triphos phates by a suitable DNA polymerase (3). The technique is especially suited for the rapid identification of chromo somes with oligonucleotide primers specific for individual chromosomal sequences and chromosomal elements like centromer or satellite sequences.

French

Domaine(s)
  • Génétique
CONT

La technique PRINS (primed in situ DNA synthesis), encore dénommée amorçage in situ, permet de localiser au niveau chromosomique des séquences répétées et présentes en de nombreuses copies. Elle nécessite une amorce nucléotidique capable de s'hybrider à la séquence d'ADN dénaturé. L'hybridation de l'amorce est suivie d'une extension in situ par incorporation, en présence de la Taq polymérase [...]

Spanish

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