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PULSED-FIELD ELECTROPHORESIS [1 record]
Record 1 - internal organization data 2012-01-12
Record 1, English
Record 1, Subject field(s)
- Molecular Biology
- Genetics
Record 1, Main entry term, English
- pulsed field gel electrophoresis
1, record 1, English, pulsed%20field%20gel%20electrophoresis
correct
Record 1, Abbreviations, English
- PFGE 2, record 1, English, PFGE
correct
Record 1, Synonyms, English
- pulsed-field gel electrophoresis 3, record 1, English, pulsed%2Dfield%20gel%20electrophoresis
correct
- PFGE 3, record 1, English, PFGE
correct
- PFGE 3, record 1, English, PFGE
- pulsed field electrophoresis 4, record 1, English, pulsed%20field%20electrophoresis
correct
- PFE 5, record 1, English, PFE
correct
- PFE 5, record 1, English, PFE
- pulsed-field electrophoresis 6, record 1, English, pulsed%2Dfield%20electrophoresis
correct
- PFE 7, record 1, English, PFE
correct
- PFE 7, record 1, English, PFE
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A method of gel electrophoresis used to separate fragments of DNA as long as several million bases by subjecting the gel to an electrical current alternately delivered from two angles in timed intervals, which minimizes diffusion of large molecules. 8, record 1, English, - pulsed%20field%20gel%20electrophoresis
Record number: 1, Textual support number: 1 CONT
Pulsed field gel electrophoresis, like other DNA electrophoresis techniques, separate DNA fragments by size, using an electrical field to force fragments to move through a gel medium. The larger fragments take longer to move through the gel, so the electrophoresis process applied must not only use alternating fields but must also last longer. 9, record 1, English, - pulsed%20field%20gel%20electrophoresis
Record 1, French
Record 1, Domaine(s)
- Biologie moléculaire
- Génétique
Record 1, Main entry term, French
- électrophorèse en champ pulsé
1, record 1, French, %C3%A9lectrophor%C3%A8se%20en%20champ%20puls%C3%A9
correct, feminine noun
Record 1, Abbreviations, French
- ECP 2, record 1, French, ECP
correct, feminine noun
Record 1, Synonyms, French
- électrophorèse sur gel en champ pulsé 3, record 1, French, %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20champ%20puls%C3%A9
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Technique utilisée pour la séparation de molécules d'ADN de haut poids moléculaire (15 à 1000 kb). 4, record 1, French, - %C3%A9lectrophor%C3%A8se%20en%20champ%20puls%C3%A9
Record number: 1, Textual support number: 1 CONT
L'électrophorèse en champ pulsé est utilisé pour séparer des fragments de taille supérieure à 20 kb. Le principe de cette électrophorèse consiste à changer l'orientation et/ou la polarité du champ électrique alternativement au cours du temps. À chaque modification du champ, la molécule d'ADN doit se réorienter parallèlement au nouveau champ. Le temps nécessaire à la réorientation est proportionnel à la longueur de la molécule. Lorsque le champ est rétabli dans son sens initial, la molécule doit une nouvelle fois se réorienter. Ces temps de réorientation provoquent un retardement de la migration nette qui est proportionnel à la taille de la molécule. Le support de migration est un gel d'agarose à 1% et la taille des fragments séparés est de l'ordre de 50 kb à qq mégabases. 5, record 1, French, - %C3%A9lectrophor%C3%A8se%20en%20champ%20puls%C3%A9
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Biología molecular
- Genética
Record 1, Main entry term, Spanish
- electroforesis en gel de campo pulsante
1, record 1, Spanish, electroforesis%20en%20gel%20de%20campo%20pulsante
correct, feminine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
Procedimiento para separar moléculas de ADN muy grandes (de 50 kpb [mil pares de bases] a varios Mpb [millón pares de bases]) alternando de forma pulsante la dirección de la corriente eléctrica a través del gel. 1, record 1, Spanish, - electroforesis%20en%20gel%20de%20campo%20pulsante
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