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SUBCLONE [1 record]
Record 1 - internal organization data 2011-06-21
Record 1, English
Record 1, Subject field(s)
- Genetics
- Biotechnology
Record 1, Main entry term, English
- subclone
1, record 1, English, subclone
correct, verb
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
There are many instances in a cell or tissue culture laboratory when the investigator is faced with the need to purify a single type of cell from a primary culture or cell line. In the case of a primary culture of tissue, for example, the heterogeneity in such a culture clearly requires cloning if one wants to study a cell comprising only a subset of the many types in the culture. In the case of a cell line, one might want to select subclones that better express the activity in question. 1, record 1, English, - subclone
Record 1, French
Record 1, Domaine(s)
- Génétique
- Biotechnologie
Record 1, Main entry term, French
- sous-cloner
1, record 1, French, sous%2Dcloner
correct
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Avec le développement de la mutagénèse dirigée sur un ADN double brin, tous les inconvénients de l'approche simple brin ont été oubliés. Plus besoin de sous-cloner ni de préparer une matrice spéciale : il suffit simplement de dénaturer chimiquement ou thermiquement l'ADN double brin avant de procéder à l'hybridation de l'oligonucléotide de mutagénèse et à la polymérisation du second brin. 1, record 1, French, - sous%2Dcloner
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
- Biotecnología
Record 1, Main entry term, Spanish
- subclonar
1, record 1, Spanish, subclonar
correct
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 CONT
Se estima que el tamaño promedio de un gen de arroz y Arabidopsis es de 2.200 pb [pares de bases]. Por otra parte, se requiere un fragmento con un tamaño mayor a 2.200 pb que permita subclonar el gen completo incluyendo posibles regiones regulatorias, para secuenciar y lograr la caracterización del gen. 1, record 1, Spanish, - subclonar
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