TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

A double-stranded DNA molecule carrying a specific sequence that will code for a given amino acid sequence and that has been produced in vitro.  3, record 1, English, - artificial%20gene

Record number: 1, Textual support number: 1 OBS

It may be synthesized using an enzyme technique to form a strand of DNA on an mRNA template using reverse transcriptase and DNA polymerase to convert the single-stranded product (cDNA) to double-stranded DNA. Alternatively, an entirely new gene may be created, which will code for a novel amino acid sequence, by using a template formed from chemically synthesized oligonucleotides followed by enzymic polymerization.  3, record 1, English, - artificial%20gene

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Gène créé par assemblage d'oligonucléotides, résultant d'une synthèse chimique, in vitro. [Définition normalisée par l'AFNOR et reproduite avec son autorisation.]  4, record 1, French, - g%C3%A8ne%20artificiel

Record number: 1, Textual support number: 1 OBS

gène artificiel; gène de synthèse; gène synthétique : termes normalisés par l'AFNOR et publiés au Journal officiel de la République française le 22 septembre 2000.  5, record 1, French, - g%C3%A8ne%20artificiel

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

A computer program, which runs on MS-DOS personal computers, is described that assists in the design of synthetic genes coding for proteins. The goal of the program is the design of a gene which (i) contains as many unique restriction sites as possible and (ii) uses a specific codon usage. The gene designed according to the criteria above is (i) suitable for 'modular mutagenesis' experiments and (ii) optimized for expression. The program 'reverse-translates' protein sequences into degenerated DNA sequences, generates a map of potential restriction sites and locates sequence positions where unique restriction sites can be accommodated. The nucleic acid sequence is then 'refined' according to a specific codon usage to remove any degeneration. Unique restriction sites, if potentially present, can be 'forced' into the degenerated nucleic acid sequence by using 'priority codes' assigned to different restriction sequences.  1, record 2, English, - synthetic%20gene

Record number: 2, Textual support number: 1 OBS

SeqAnalRef. Sequence Analysis Bibliographic Reference Data Bank. Release: 67.0 / February 1996 in Internet at the address mentioned in b.  2, record 2, English, - synthetic%20gene

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

[...] les chimistes ont déjà créé des molécules capables de s'assembler et de se répliquer elles-mêmes - et de nouveaux types de microorganismes, totalement différents de ceux qui existent aujourd'hui. Ceux-ci intégreront des gènes synthétiques et seront améliorés par sélection accélérée, selon le principe des mécanismes naturels de l'évolution.  1, record 2, French, - g%C3%A8ne%20synth%C3%A9tique

Record 2,  Textual support,  Spanish