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TISSUE CULTURE PROPAGATION [1 record]

Record 1 2003-06-05

English

Subject field(s)
  • Scientific Research
  • Plant Breeding
  • Bioengineering
  • Biotechnology
DEF

The propagation of plants in a controlled, artificial environment, using plastic or glass culture vessels, aseptic techniques, and a defined growing medium.

CONT

The procedures used for in vitro propagation comprise : (1) the selection of suitable explants, their sterilisation and transfer to nutrient medium, (2) the proliferation of shoots on multiplication medium and (3) the transfer of shoots to a rooting (or storage) medium and planting out.

CONT

The invention provides a process for a least semi-automated plant tissue culture propagation comprising: providing plant tissue culture containing a large number of densely packed meristematic areas, automatically separating and thinning the tissue into small clusters of meristematic tissues, bulk transporting the separated tissue aseptically to a separating apparatus wherein the tissue is washed and propagules of substantially uniform size and substantially free of phytotoxic cell debris are obtained, aseptically transporting the propagules to a culture vessel and distributing the propagules on the surface of a culture medium.

French

Domaine(s)
  • Recherche scientifique
  • Amélioration végétale
  • Technique biologique
  • Biotechnologie
DEF

Technique de propagation des plantes en tubes à essai, couramment utilisée dans le but d'obtenir des plantes saines ou pour avoir rapidement des plantes identiques à la plante d'origine.

CONT

La technique de micropropagation comprend les étapes suivantes : 1) sélection d'un explant propice, stérilisation et le transfert dans un milieu nutritif, 2) prolifération des pousses et multiplication en culture aseptique, 3) transfert des pousses à un milieu propice à l'enracinement et mise en serre.

Spanish

Campo(s) temático(s)
  • Investigación científica
  • Mejora vegetal
  • Bioingeniería
  • Biotecnología
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