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X-RAY MICROSCOPY [1 record]

Record 1 2014-02-07

English

Subject field(s)
  • Radiological Physics (Theory and Application)
CONT

High brightness, short pulse width (<200 picoseconds) x-ray lasers now offer biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motion or chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at a 44.83-angstrom wavelength was used together with an x-ray zone plate optic to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. In the image, 500-angstrom features are clearly visible. This illustrates the importance of x-ray microscopy in determining chemical composition. X-ray microscopy also provides information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

French

Domaine(s)
  • Physique radiologique et applications
OBS

La microscopie par rayons X utilise des «X mous» d'une longueur d'onde comprise entre 1 et 10 nm.

Spanish

Campo(s) temático(s)
  • Física radiológica (Teoría y aplicación)
CONT

La microscopía de rayos X ha emergido como técnica para observar estructuras que no son accesibles con microscopía óptica convencional, y que tiene ventajas respecto a la microscopía electrónica debido a la mayor longitud de penetración y a la sensibilidad química de la radiación X.

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