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AMORCAGE IN SITU [1 record]
Record 1 - internal organization data 1997-05-07
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- primed in situ labeling of nucleic acids
1, record 1, English, primed%20in%20situ%20labeling%20of%20nucleic%20acids
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- primed in situ labeling 2, record 1, English, primed%20in%20situ%20labeling
correct
- PRINS 3, record 1, English, PRINS
correct
- PRINS 3, record 1, English, PRINS
- primed in situ DNA synthesis 4, record 1, English, primed%20in%20situ%20DNA%20synthesis
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Primed in situ labeling (PRINS) is a fast alternative to traditional in situ hybridization, requiring only a tew handling steps (1,2). In the PRINS reaction, an unlabeled sequence-specific oligonucleotide primer or short DNA fragment is hybridized (annealed) to denatures chromosomal DNA and extended in situ in the presence of hapten-labeled nucleotide triphos phates by a suitable DNA polymerase (3). The technique is especially suited for the rapid identification of chromo somes with oligonucleotide primers specific for individual chromosomal sequences and chromosomal elements like centromer or satellite sequences. 2, record 1, English, - primed%20in%20situ%20labeling%20of%20nucleic%20acids
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- technique PRINS
1, record 1, French, technique%20PRINS
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- technique de PRINS 2, record 1, French, technique%20de%20PRINS
correct, feminine noun
- amorçage in situ 1, record 1, French, amor%C3%A7age%20in%20situ
correct, masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La technique PRINS (primed in situ DNA synthesis), encore dénommée amorçage in situ, permet de localiser au niveau chromosomique des séquences répétées et présentes en de nombreuses copies. Elle nécessite une amorce nucléotidique capable de s'hybrider à la séquence d'ADN dénaturé. L'hybridation de l'amorce est suivie d'une extension in situ par incorporation, en présence de la Taq polymérase [...] 1, record 1, French, - technique%20PRINS
Record 1, Spanish
Record 1, Textual support, Spanish
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