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DIG-LABELED HYBRIDIZATION ASSAY [1 record]
Record 1 - internal organization data 1996-11-05
Record 1, English
Record 1, Subject field(s)
- Biotechnology
- Crop Protection
Record 1, Main entry term, English
- digoxygenin-labeled hybridization assay 1, record 1, English, digoxygenin%2Dlabeled%20hybridization%20assay
Record 1, Abbreviations, English
- dig-labeled hybridization assay 2, record 1, English, dig%2Dlabeled%20hybridization%20assay
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
A dig-labeled hybridization assay was developed for the detection of crown gall disease (Agrobacterium vitis), providing a highly sensitive, specific, and low-cost alternative to polymerase chain reaction assays. 1, record 1, English, - digoxygenin%2Dlabeled%20hybridization%20assay
Record 1, French
Record 1, Domaine(s)
- Biotechnologie
- Protection des végétaux
Record 1, Main entry term, French
- épreuve d'hybridation par marquage à la digoxygénine
1, record 1, French, %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- test d'hybridation par marquage à la digoxygénine 1, record 1, French, test%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
masculine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 OBS
L'hybridation consiste à mettre en évidence, grâce au marquage, l'appariement d'une molécule d'acide nucléique sonde avec une molécule d'acide nucléique cible dont la séquence est complémentaire. La mise au point de techniques de marquage non radioactif performantes (sondes froides) permet d'envisager une large application de l'hybridation moléculaire pour la détection des agents phytopathogènes. 2, record 1, French, - %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
Record number: 1, Textual support number: 2 OBS
De nombreux haptènes ont été employés avec succès pour préparer des sondes non radioactives : biotine, éthidium, N-2-acétyl-aminofluorène (AAF), triphénol, sulfone, digoxygénine ... Après hybridation, la sonde est détectée grâce à une protéine marquée spécifique de cet haptène. [...] Il existe plusieurs schémas réactionnels disponibles pour réaliser un test d'hybridation moléculaire. 2, record 1, French, - %C3%A9preuve%20d%27hybridation%20par%20marquage%20%C3%A0%20la%20digoxyg%C3%A9nine
Record 1, Spanish
Record 1, Textual support, Spanish
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