TERMIUM Plus®

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GRAM REACTION [1 record]

Record 1 2008-12-10

English

Subject field(s)
  • Diagnostic Procedures (Medicine)
  • Microbiology and Parasitology
  • Bacterial Diseases
DEF

A method for the differential staining of bacteria by treatment with a watery solution of iodine and the iodide of potassium after staining with a triphenylmethane dye (as crystal violet).

CONT

The Gram staining method ... is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria ... The microorganisms that retain the crystal violet-iodine complex appear purple brown under microscopic examination [and] are commonly classified as gram positive ... Others that are not stained by crystal violet are referred to as gram negative.

French

Domaine(s)
  • Méthodes diagnostiques (Médecine)
  • Microbiologie et parasitologie
  • Maladies bactériennes
DEF

Technique de coloration différentielle distinguant les bactéries Gram-positives et Gram-négatives sur la base de leur capacité à retenir le crystal violet lorsqu'elles sont décolorées à l'aide d'un solvant organique comme l'éthanol.

Spanish

Campo(s) temático(s)
  • Procedimientos diagnósticos (Medicina)
  • Microbiología y parasitología
  • Enfermedades bacterianas
DEF

Método de tinción diferencial de bacterias; los frotis se fijan a la llama, se tiñen con una solución de violeta cristal, se trata con solución de yodo, se lavan, se decoloran y luego se colorean por contraste con safranina O. Los microorganismos grampositivos se tiñen de negro violáceo y los gramnegativos de rosado; es útil en taxonomía e identificación bacteriana y también para indicar diferencias fundamentales en la estructura de la pared celular.

OBS

Yodo de Gram: solución que contiene yodo y yoduro de potasio, usada en el colorante de Gram.

OBS

Colorante de Gram: yodo, 1 g; yoduro potásico, 2 g; agua, 300 ml.

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