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HYBRID-RELEASED TRANSLATION [1 record]

Record 1 2011-01-28

English

Subject field(s)
  • Genetics
DEF

Method that enables a cloned DNA to be correlated with the protein for which it encodes.

OBS

Cloned DNA is bound to a cellulose nitrate filter and hybridized with an unfractionated preparation of mRNA. The filter is washed and the hybridized mRNA eluted by heating in a buffer with formamide. The recovered mRNA is then translated in a cell-free system, and the radiolabelled polypeptide products analyzed by gel electrophoresis.

French

Domaine(s)
  • Génétique

Spanish

Campo(s) temático(s)
  • Genética
DEF

Método utilizado para identificar el producto génico de un gen clonado.

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