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OVERLAY ASSAY [1 record]

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Record 1 2011-11-02

English

Subject field(s)
  • Bioengineering
  • Molecular Biology
CONT

A far-Western blot (also known as an overlay assay) is used to detect the interaction of a recombinant GST [glutathione S-transferase] fusion protein (produced and purified from bacteria) with a target protein on a membrane.

French

Domaine(s)
  • Technique biologique
  • Biologie moléculaire
CONT

L'utilisation de la technique de Far-Western, dans le but d'identifier les interactions protéine-protéine entre un virus et son vecteur, ont permis d'isoler deux protéines de thrips de 50 et 94 kDa [kiloDaltons] présentant des affinités particulières pour les glycoprotéines du TSWV [tomato spotted wilt virus].

Spanish

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