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SPECTRAL KARYOTYPING [2 records]
Record 1 - internal organization data 1999-10-22
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- spectral karyotyping
1, record 1, English, spectral%20karyotyping
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 OBS
The new karyotyping methods introduced by Schrock et al use fluorescent dyes that bind to specific regions of chromosomes. By using a series of specific probes each with varying amounts of the dyes, different pairs of chromosomes have unique spectral characteristics. A unique feature of the technology is the use of an interferometer similar to ones used by astronomers for measuring light spectra emitted by stars. Slight variations in color, undetectable by the human eye, are detected by a computer program that then reassigns an easy-to-distinguish color to each pair of chromosomes. The result is a digital image rather than film, in full color. Pairing of the chromosomes is simpler because homologous pairs are the same color, and aberrations and cross-overs are more easily recognizable. In additional, the spectral karyotype has been used to detect translocations not recognizable by traditional banding analysis. 1, record 1, English, - spectral%20karyotyping
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- caryotype spectral
1, record 1, French, caryotype%20spectral
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Par isolement de chacune des paires chromosomiques, en cytométrie de flux ou à l'aide de la microdissection, la FISH est désormais capable de réaliser de vieux rêves que les cytogénéticiens jugèrent longtemps puérils et utopiques : le coloriage du caryotype en un ravissant nuancier (le caryotype spectral) où chaque paire devient identifiable et à partir duquel toute reconnaissance (de remaniements, chromosomes marqueurs, fragments surnuméraires...) devient possible. 1, record 1, French, - caryotype%20spectral
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 1997-07-16
Record 2, English
Record 2, Subject field(s)
- Genetics
Record 2, Main entry term, English
- spectral karyotyping
1, record 2, English, spectral%20karyotyping
correct
Record 2, Abbreviations, English
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 OBS
The new karyotyping methods introduced by Schrock et al use fluorescent dyes that bind to specific regions of chromosomes. By using a series of specific probes each with varying amounts of the dyes, different pairs of chromosomes have unique spectral characteristics. A unique feature of the technology is the use of an interferometer similar to ones used by astronomers for measuring light spectra emitted by stars. Slight variations in color, undetectable by the human eye, are detected by a computer program that then reassigns an easy-to-distinguish color to each pair of chromosomes. The result is a digital image rather than film, in full color. Pairing of the chromosomes is simpler because homologous pairs are the same color, and aberrations and cross-overs are more easily recognizable. In additional, the spectral karyotype has been used to detect translocations not recognizable by traditional banding analysis. 1, record 2, English, - spectral%20karyotyping
Record 2, French
Record 2, Domaine(s)
- Génétique
Record 2, Main entry term, French
- caryotype spectral
1, record 2, French, caryotype%20spectral
correct, masculine noun
Record 2, Abbreviations, French
Record 2, Synonyms, French
Record 2, Textual support, French
Record number: 2, Textual support number: 1 OBS
Nouvelle technique de cytogénétique moléculaire. 2, record 2, French, - caryotype%20spectral
Record number: 2, Textual support number: 1 DEF
Méthode fondée sur la discrimination de chaque fluorochrome via l'établissement d'un spectre d'émission de fluorescence (environ 50 à 100 valeurs utiles) et chaque point de l'image. Cette approche la différencie de la microscopie en épifluorescence classique où la détermination des fluorochromes est effectuée par le biais d'une seule mesure [...] 1, record 2, French, - caryotype%20spectral
Record number: 2, Textual support number: 1 CONT
Le caryotype spectral pourrait être rapidement inclus de façon complémentaire dans les protocoles cytogénétiques hospitaliers d'étude des cancers. 1, record 2, French, - caryotype%20spectral
Record number: 2, Textual support number: 2 CONT
Le caryotype spectral, décrit par l'équipe de T Ried au National Institute Of Health (Bethesda) en juin 1996, est une démonstration extrêmement convaincante que chaque chromosome est identifiable spécifiquement et simultanément par sa coloration en peinture chromosomique. La puissance de cette méthode permet «in fine» de caractériser des remaniements chromosomiques de structure complexe, même en l'absence de bandes. 3, record 2, French, - caryotype%20spectral
Record 2, Spanish
Record 2, Textual support, Spanish
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