TERMIUM Plus^®

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Record number: 1, Textual support number: 1 OBS

Heating DNA molecules to temperatures just below 100 [degrees] C breaks the hydrogen bonds holding the complementary strands together; they then quickly separate from each other (DNA denaturation). If the temperature is gradually lowered, the complementary strands again form the correct hydrogen bonds and the double-helical form is regained (renaturation of DNA). If, however, this gentle cooling is done in the presence of single-stranded RNA that has been synthesized on homologous DNA, then DNA-RNA hybrids form as well as denatured DNA double helices. These DNA-RNA hybrids are very specific and form only if some of the nucleotide sequences in the DNA are complementary to the RNA nucleotide sequences.  1, fiche 1, Anglais, - renatured%20DNA

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