TERMIUM Plus^®

Fiche 1,  Justifications,  Anglais

Record number: 1, Textual support number: 1 CONT

Replica filters are made as follows: A. Prepare in advance a stack of sterile Whatman 3MM filters (one for each filter plus a few spares). B. Peel the master nitrocellulose filter off the storage plate and lay it colony side up on a dampened pad of sterile, 3MM filter paper. C. Number a damp, sterile nitrocellulose filter and lay it on the master nitrocellulose filter. D. Press the two filters firmly together with a velvet replica-plating tool. E. Orient the two filters by placing a series of holes made in the pair of filters with an 18-gauge needle. F. Peel apart the filters. Lay the replica on a fresh agar plate and incubate at 37 ° C until colonies appear. G. Replace the master nitrocellulose filter on a fresh agar plate containing 25% glycerol. Incubate for 1 hour at 37 ° C and then freeze as described in step 6 above.  1, fiche 1, Anglais, - replica%20filter

Fiche 1,  Justifications,  Français

Fiche 1,  Justifications,  Espagnol