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LYSIS BUFFER [1 record]

Record 1 1998-12-02

English

Subject field(s)
  • Biological Sciences
CONT

Once a snake was captured we drew ca. 100 ul of blood from a caudal vessel using an insulin syringe and blood samples were immediately mixed with lysis buffer and refrigerated until DNA extraction.

French

Domaine(s)
  • Sciences biologiques
CONT

Les fractions membranaire et cytosolique des PMNL sont séparées par une sonication dans un tampon de lyse. L'activité de la PKC est mesurée suite à l'incorporation de 32P dans un substrat synthétique.

CONT

L'activité lytique de la fraction LE III b est optimale à la basse concentration de 0,01 M dans le tampon de lyse, et chute en présence de Hg Cl SUB 2 et d'EDTA.

Spanish

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