TERMIUM Plus^®

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A growth factor with specificity for vascular endothelial cells has been identified in conditioned medium of pituitary-derived growth factor(FSdGF), was purified to homogeneity by a combination of heparin-Sepharose affinity chromatography, Bio-Gel P-60 exclusion chromatography, Mono Sion-exchange chromatography, and hydrophobic chromatography on a C4 reverse-phase HPLC column. FSdGF(folliculo stellate growth factor) was a molecular mass of 23 KDa...  3, fiche 1, Anglais, - folliculo%20stellate%20derived%20growth%20factor

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Tiré de: Proceedings of the National Academy of Sciences of USA, 86 (19), 1989, 7311-7315.  3, fiche 1, Anglais, - folliculo%20stellate%20derived%20growth%20factor

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La vasculotropine est le dernier facteur angiogénique découvert. Il a été isolé à partir de cellules folliculaires de l'hypophyse de bœuf ou à partir du milieu conditionné de la lignée cellulaire hypophysaire AtT-20 (J. Plouet, communication personnelle). Il porte également le nom de folliculo stellate-derived growth factor (FSdGF).  1, fiche 1, Français, - vasculotropine

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Fiche 2,  Justifications,  Anglais

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The "shake flask" method described is based on the OECD [Organisation for Economic Co-operation and Development] Test Guideline [i. e. the 1981 Test Guideline 107. ] This test method includes two separate procedures : the shake-flask method and high performance liquid chromatography(HPLC).... The shake-flask method applies only to essentially pure substances soluble in water and n-octanol. It is not applicable to surface active materials(for which a calculated value or an estimate based on the individual n-octanol and water solubilities should be provided).  2, fiche 2, Anglais, - shake%2Dflask%20method

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Il existe une méthode normalisée (flacon d’agitation) qui permet de déterminer le POW [POE, ou coefficient de partage 1-n-octanol/eau] à savoir les Lignes directrices de l'OCDE [Organisation de coopération et de développement économiques pour les essais de produits chimiques — Essai n° 107, 1981.] La méthode du flacon d’agitation est applicable aux substances solubles dans l’eau, et qui ne se dissocient pas ni ne s’associent. Cette méthode n’est pas applicable aux substances organiques lipophiles (à faible solubilité dans l’eau), aux préparations, aux substances complexes, aux composés organométalliques et aux substances tensioactives. Cette méthode permet de mesurer des valeurs de log [Poe] se situant entre – 2 et 4.  2, fiche 2, Français, - m%C3%A9thode%20par%20agitation%20en%20flacon

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Liquid chromatography–mass spectrometry(LC-MS, or alternatively HPLC-MS) isan analytical chemistry technique that combines the physical separation capabilities of liquid chromatography(or HPLC) with the mass analysis capabilities of mass spectrometry(MS). LC-MS is a powerfultechnique that has very high sensitivity and selectivity and so is useful in many applications. Its application is oriented towards the separation, general detection and potential identification of chemicals of particular masses in the presence of other chemicals[. ]  4, fiche 3, Anglais, - quadrupole%20time%2Dof%2Dflight%20liquid%20chromatography%2Dmass%20spectrometry

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There are many different mass analyzers that can be used in LC/MS. Single quadrupole, triple quadrupole, ion trap, time of flight (TOF) and quadrupole-time of flight (Q-TOF).  4, fiche 3, Anglais, - quadrupole%20time%2Dof%2Dflight%20liquid%20chromatography%2Dmass%20spectrometry

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La chromatographie en phase liquide couplée à la spectrométrie de masse (en anglais Liquid chromatography-mass spectrometry ou LC-MS) est une méthode d'analyse qui combine les performances de la chromatographie en phase liquide et de la spectrométrie de masse afin d'identifier et/ou de quantifier précisément de nombreuses substances.  3, fiche 3, Français, - chromatographie%20liquide%2Dspectrom%C3%A9trie%20de%20masse%20quadripolaire%20%C3%A0%20temps%20de%20vol

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A device used to detect fluorescent compounds in high-pressure liquid chromatography [HPLC] by passing the effluent through a cell irradiated with ultraviolet light and measuring any resulting fluorescent radiation.  3, fiche 4, Anglais, - fluorimetric%20detector

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Fixed band-pass fluorometric detector.  3, fiche 4, Anglais, - fluorimetric%20detector

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fluorimetric detector: term(s) extracted from the "Disarmament and Peace Keeping" glossary with the permission of the United Nations Office at Geneva.  4, fiche 4, Anglais, - fluorimetric%20detector

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détecteur fluorimétrique : terme(s) tiré(s) du lexique «Désarmement et Maintien de la Paix» avec l'autorisation de l'Office des Nations Unies à Genève.  2, fiche 4, Français, - d%C3%A9tecteur%20fluorim%C3%A9trique

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Fiche 5,  Justifications,  Anglais

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Due to the drop in pressure that occurs in high-pressure liquid chromatography(HPLC) as the solvent passes from the column to the detector, any dissolved gases(most commonly air) are partially released as bubbles within the detector, producing anomalous and misleading results. To avoid this problem, solvents are degassed before use, either by a nitrogen purge or by heating and stirring under vacuum.  2, fiche 5, Anglais, - degassing

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The process of solute movement into and out of the stationary phase or mobile phase. It is represented by the C term of the van Deemter equation and is referred to as the mass transfer term. The faster the process of mass transfer, the better the efficiency of the column. In HPLC, mass transfer is the most important factor affecting column efficiency. It is increased by the use of small particle packings, thin layers of stationary phase, low viscosity mobile phases, and high temperatures.  3, fiche 6, Anglais, - mass%20transfer%20term

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By the fall of 1996, LCDC anticipates offering the services of HPLC of bacterial cell wall components to corroborate bacterial taxonomy. This will include corynomycolates, mycolates, isoprenoid quinones, menaquinones, and other components.  2, fiche 7, Anglais, - mycolate

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Conventional identification characteristics, mycolate and fatty acid composition, and clinical significance of MAIX accuprobe-positive isolates of Mycobacterium avium complex.  3, fiche 7, Anglais, - mycolate

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The initial solubilization of yolk platelets demonstrated that the lipid was present in the soluble fraction containing lipovitellin and phosvitin. These two proteins have been resolved and the lipovitellin fraction was shown to exhibit the absorption maximum at 375 nm that characterizes DCD [dorsalizing cytoplasmic determinant]. The lipovitellin was purified and its total lipids extracted and separated on HPLC [high-performance liquid chromatography]. A lipid was found that has identical elution properties from a C18 reverse HPLC column and mass as DCD. Therefore, lipovitellin, a zinc protein known to contain 17% lipid by weight, is the oocyte storage protein for DCD.  2, fiche 8, Anglais, - lipovitellin

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The use of HPLC [high performance liquid chromatography] to determine the saturate content of heavy petroleum products.... The refractive index response factors for several pure saturate fractions isolated from heavy petroleum products were determined using a µ-Bondapak aminopropylmethylsilyl bonded amorphous silica HPLC column.  2, fiche 9, Anglais, - aminopropylmethylsilyl%20bonded%20amorphous%20silica

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Column - Water carbohydrate analysis [trademark] partition column. Aminopropylmethylsilyl bonded amorphous silica, 300 mm x 3.9 mm (ID) or equivalent.  3, fiche 9, Anglais, - aminopropylmethylsilyl%20bonded%20amorphous%20silica

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Ultraviolet-Visible Detectors. Optical detectors based on ultraviolet-visible absorption constitute over 70 percent of HPLC detection systems. It is not necessary that the wavelength selected for HPLC coincide with the wavelength where waximum absorption occurs.... Detectors are available that span the full range from single-wavelength to simultaneous multiwavelength detection capabilities.  1, fiche 10, Anglais, - ultraviolet%2Dvisible%20absorption

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ultraviolet : Cette graphie, puisée des Rectifications de l'orthographe recommandées par le Conseil supérieur de la langue française, est attestée dans le Petit Robert (2006).  2, fiche 10, Français, - absorption%20par%20ultraviolet%20visible

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Fiche 11,  Justifications,  Anglais

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Ultraviolet-visible detectors. Optical detectors based on ultraviolet-visible absorption constitute over 70 percent of HPLC detection systems. It is not necessary that the wavelength selected for HPLC coincide with the wavelength where waximum absorption occurs.... Detectors are available that span the full range from single-wavelength to simultaneous multiwavelength detection capabilities.  1, fiche 11, Anglais, - ultraviolet%2Dvisible%20detector

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ultraviolet : Cette graphie, puisée des Rectifications de l'orthographe recommandées par le Conseil supérieur de la langue française, est attestée dans le Petit Robert (2006).  2, fiche 11, Français, - d%C3%A9tecteur%20%C3%A0%20l%27ultraviolet%20visible

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The most common product used for HPLC [high-performance liquid chromatography] samples is the Cameo nylon syringe filter... The Cameo polyethersulfone syringe filters, sterile or non-sterile, are also used for biological fluids, serum or media additives. This low protein binding filter is often used where quick flow rates and high throughputs are critical.  2, fiche 12, Anglais, - low%20protein%2Dbinding%20filter

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Procédure de marquage. Préparation du réactif et détermination de la dilution de travail: [...] Des particules sont susceptibles d'apparaître au cours de la conservation et d'interférer avec l'interprétation des résultats. La dilution de travail peut être distribuée sur la lame au moyen d'une seringue sur laquelle a été adapté un filtre à faible liaison aux protéines de 0,45 µm. Éviter de répéter les étapes de filtration ou de centrifugation. La dilution de travail adsorbée avec du NMB ou du RMB ne doit pas être filtrée.  2, fiche 12, Français, - filtre%20%C3%A0%20faible%20liaison%20aux%20prot%C3%A9ines

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filtre à faible liaison aux protéines : terme retenu par le réseau Entraide Traduction Santé.  3, fiche 12, Français, - filtre%20%C3%A0%20faible%20liaison%20aux%20prot%C3%A9ines

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A chromatographic technology used to separate and quantitate mixtures of substances in solution.  5, fiche 13, Anglais, - high%2Dperformance%20liquid%20chromatography

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The sample is injected into a moving stream of solvent, which flows through a column and detector. The passage through the column separates the sample compounds by one of four different methods: adsorption, partition, ion exchange, or size exclusion. The solvent is pumped through the system at a controlled pressure, which can be varied between 500 and 5000 psi. With the particular column and elution method used for the assay, the elution times relative to the internal standard characterize the compounds and are used to identify them. The peak areas relative to the internal standard are proportional to their concentration in the sample and are used to quantitate them.  6, fiche 13, Anglais, - high%2Dperformance%20liquid%20chromatography

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A special form of liquid chromatography, high-performance liquid chromatography(HPLC), has become especially important for the separation of complex mixtures of nonvolatile materials. Essentially, it involves the use of very small particle size in column packing, with high pressure to increase the rate of flow and shorten the separation time to a few minutes.  7, fiche 13, Anglais, - high%2Dperformance%20liquid%20chromatography

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One of the most significant advances in chromatography in recent years has been the introduction of high performance liquid chromatography. Originally intended for the separation of small organic molecules that were soluble in non-aqueous solvents, the technique rapidly developed into a form suitable for the separation of larger, biological compounds soluble in aqueous solvents. In recent years, following the development of macroporous supports, the technique has become applicable to molecules as large as proteins and nucleic acids.  8, fiche 13, Anglais, - high%2Dperformance%20liquid%20chromatography

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The term high pressure liquid chromatography (now obsolete) was used in the early stages of development of the technique since the quality of the columns was such that high pressures were needed to force the liquid through.  4, fiche 13, Anglais, - high%2Dperformance%20liquid%20chromatography

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Technique qui permet d'obtenir une séparation de mélanges complexes selon le même principe que la chromatographie liquide classique (échange d'ions, tamisage moléculaire, ou interaction hydrophobe) mais en des temps très courts grâce à l'emploi de hautes pressions.  12, fiche 13, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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Les avantages de l'HPLC [«high-performance liquid chromatography»] que sont la rapidité et la possibilité d'automatiser totalement l'analyse et le traitement des données (mise en mémoire et traitement informatique des courbes chromatographiques), sont susceptibles de conduire cette technique à remplacer, au moins dans certaines applications, l'électrophorèse classique actuelle.  12, fiche 13, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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La chromatographie liquide à haute performance (HPLC) est une méthode récente. L'évolution technologique a permis de réaliser des résines sphériques de taille allant jusqu'à 5 microns, ce qui leur donne des propriétés mécaniques telles qu'elles supportent de forts débits ainsi que la haute contre-pression qui en résulte. On réalise ainsi des séparations beaucoup plus rapides avec une meilleure résolution. L'appareil est constitué d'une colonne en acier inoxydable d'une longueur de l'ordre de 200 mm et de diamètre intérieur de 4 mm, remplie de particules de taille inférieure à 20 mm; cette colonne est précédée d'une pompe à haute pression, permettant d'injecter le solvant sous une pression de plusieurs dizaines de bars. Des microvannes assurent l'introduction de quelques microlitres de solution à analyser, en tête de colonne.  13, fiche 13, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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Chromatographie liquide à haute performance multidimensionnelle.  14, fiche 13, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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CLAR es una técnica analítica en la que una fase móvil líquida transporta una muestra a través de una columna que contiene una fase líquida estacionaria. La interacción de la muestra con la fase estacionaria retiene de modo selectivo los compuestos individuales y permite la separación de los componentes de la muestra [...]  2, fiche 13, Espagnol, - cromatograf%C3%ADa%20l%C3%ADquida%20de%20alta%20presi%C3%B3n

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HPLC separations and quantitative analyses are described for palmitic and soya monoethanolamides and diethanolamides synthesized from free fatty acids, methyl esters and triglycerides.  1, fiche 14, Anglais, - diethanolamide

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HPLC separations and quantitative analyses are described for palmitic and soya monoethanolamides and diethanolamides synthesized from free fatty acids, methyl esters and triglycerides.  1, fiche 15, Anglais, - monoethanolamide

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To obtain the rapid separation of large quantities of materials, various techniques have been developed to produce continuous forms of chromatography. The easiest of these is the batch process of automatic sampling and fraction collecting applied regularly to GC [gas chromatography] and HPLC [high pressure liquid chromatography]. Other more truly continuous forms of chromatography have been developed for GC, including the use of circular columns, moving bed processes and radial flow columns.  1, fiche 16, Anglais, - continuous%20chromatography

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Protein purification development and assay : Retentate Chromatography is a new SELDID development which allows rapid protein separation of complex biological fluids on ProteinChip arrays using the bioprocessor accessory for controlled washing of different chromatographically prepared surfaces(anion, cation, IMAV, normal & reversed phase). Retentate mapping’ has many advantages over 2-D gel electrophoresis and other elution chromatography methods for speeding purification protocol development as well as replacing Western Blot, HPLC and ELISA assays.  1, fiche 17, Anglais, - bioprocessor

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Affymetrix a déjà mis au point un produit qu'elle commercialise sous l'appellation «GeneChip», sorte de d'équivalent biologique du microprocesseur, destiné à satisfaire précisément ce genre de besoin. De quoi s'agit-il? Un faisceau lumineux est dirigé sur la surface du processeur biologique pour activer les régions qui recevront les sondes ADN. Celles-ci sont construites sur la surface et s'assemblent vers l'extérieur à mesure que les bases sont ajoutées. Des marqueurs fluorescents incorporés à l'ADN cible permettent de déterminer s'il y a hybridation avec les sondes complémentaires.  1, fiche 17, Français, - processeur%20biologique

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Supplément à La Recherche.  2, fiche 17, Français, - processeur%20biologique

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Technique in which the mobile phase(fluid) is maintained at temperatures somewhat above its critical point. The mobile phases used in SFC are gases such as freon, ethylene, or carbon dioxide. It has superior solution properties and enhances the chromatographic separation of higher molecular weight compounds. The column packings used in SFC are the same as those used in HPLC.  2, fiche 18, Anglais, - supercritical%20fluid%20chromatography

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By the fall of 1996, LCDC anticipates offering the services of HPLC of bacterial cell wall components to corroborate bacterial taxonomy. This will include corynomycolates, mycolates, isoprenoid quinones, menaquinones, and other components.  1, fiche 19, Anglais, - isoprenoid%20quinone

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quinones: aromatic dioxo compounds derived from benzene or multiple-ring hydrocarbons such as naphtalene, anthracenes, etc.  2, fiche 19, Anglais, - isoprenoid%20quinone

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By the fall of 1996, LCDC anticipates offering the services of HPLC of bacterial cell wall components to corroborate bacterial taxonomy. This will include corynomycolates, mycolates, isoprenoid quinones, menaquinones, and other components.  1, fiche 20, Anglais, - corynomycolate

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Les énolates de lithium des propionates de diméthil-2,5 phényle et de di-t-butyl-2,6 P-tolyle se condensent avec des aldéhydes (benzaldéhyde, alcanals) et donnent de façon prédominante les thréo-aldols. Par hydrolyse de ces aldols, obtention de beta-hydroxyamides. Application à la synthèse stéréosélective du corynomycolate de méthyle.  1, fiche 20, Français, - corynomycolate

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Traduction du résumé d'un article paru dans le périodique suivant : Journal: Tétrahedron, 1981. 37 (23) 4087-4095.  1, fiche 20, Français, - corynomycolate

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Method involving a chromatographic system such as GLC or HPLC connected to a mass spectrometer of FTIR spectrometer in order to analyze individual components from a mixture of organic compounds in minute(submilligram) quantities.  1, fiche 21, Anglais, - coupled%20chromatographic%20and%20spectroscopic%20techniques

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For conventional HPLC conditions, typical detectability with commercial absorbance detectors is 1 ng of injected analyte...  1, fiche 22, Anglais, - absorbance%20detector

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The amperometric detector is useful for detecting electroactive substances and has found considerable use in biological applications, for example, in the HPLC separation and detection of trace quantities of catecholamines from the brain.  2, fiche 23, Anglais, - amperometric%20detector

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[...] les détecteurs ampérométriques [...] n'effectuent qu'une électrolyse partielle du soluté. Les détecteurs ampérométriques sont, d'une façon générale, équipés d'une cellule de très faible volume mort [...] et des limites de détectabilité voisines de 10^{minus 10} g de substance injectée sont couramment atteintes pour de nombreux composés organiques.  1, fiche 23, Français, - d%C3%A9tecteur%20amp%C3%A9rom%C3%A9trique

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Fluorescence detection in HPLC provides excellent selectivity and sensitivity.... If the detector will be used exclusively for a specific task and the chromatography is fairly clean, a filter fluorometer is quite appropriate.  1, fiche 24, Anglais, - filter%20fluorometer

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Fluorescence detection in HPLC provides excellent selectivity and sensitivity.  1, fiche 25, Anglais, - fluorescence%20detection

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Ultraviolet-Visible Detectors. Optical detectors based on ultraviolet-visible absorption constitute over 70 percent of HPLC detection systems. It is not necessary that the wavelength selected for HPLC coincide with the wavelength where waximum absorption occurs.... Detectors are available that span the full range from single-wavelength to simultaneous multiwavelength detection capabilities.  2, fiche 26, Anglais, - optical%20detector

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The essential components of HPLC... components include : A pump or pumps to force the mobile phase through the system. Suitable pressure gauges and flow meters are placed in the system.  1, fiche 27, Anglais, - pump

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[...] la perte de charge, inversement proportionnelle au diamètre des particules augmente elle aussi rapidement, pouvant même devenir supérieure à la pression maximale de refoulement de la pompe du chromatographe.  1, fiche 27, Français, - pompe

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A potent new approach which marries the inherent speed and resolving power of HPLC with the biological specificity of affinity chromatography.  1, fiche 28, Anglais, - high%20performance%20liquid%20affinity%20chromatography

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The technique of HPLAC was used to resolve a mixture of horse liver alcohol dehydrogenase and pig heart lactate dehydrogenase by consecutive elution with appropriate ternary complexes.... The two enzymes were separated ... in near quantitative yield and in less than 5 minutes. Furthermore, the heart muscle (H4) and skeletal muscle (M4) isoenzymes of lactate dehydrogenase were also resolved with equal rapidity on the AMP-silica column by a gradient of NADH.  3, fiche 28, Anglais, - high%20performance%20liquid%20affinity%20chromatography

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The general versatility of HPLAC has been amply demonstrated with the resolution of enzymes and isoenzymes, albumin, protein antigens and antibodies, carbohydrates, glycoproteins, nucleosides and nucleotides using microparticulate silica-immobilized nucleotides, boronates, immunoadsorbents, lectins and charge transfer ligands.  1, fiche 28, Anglais, - high%20performance%20liquid%20affinity%20chromatography

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L'apparition de supports rigides facilement dérivables par les ligands va inévitablement avoir pour conséquence le développement de la chromatographie d'affinité sous pression. Le biologiste va ainsi bénéficier de la puissance de l'association affinité-pression : - vitesse accrue de chargement de l'échantillon sur la colonne; (...) - temps de lavage de la colonne; (...) - vitesse d'évolution rapide : de l'ordre de quelques minutes seulement. A ces avantages, il faut ajouter que ces nouveaux supports ont une longévité importante.  1, fiche 28, Français, - chromatographie%20d%27affinit%C3%A9%20sous%20pression

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