TERMIUM Plus^®

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A growth factor with specificity for vascular endothelial cells has been identified in conditioned medium of pituitary-derived growth factor(FSdGF), was purified to homogeneity by a combination of heparin-Sepharose affinity chromatography, Bio-Gel P-60 exclusion chromatography, Mono Sion-exchange chromatography, and hydrophobic chromatography on a C4 reverse-phase HPLC column. FSdGF(folliculo stellate growth factor) was a molecular mass of 23 KDa...  3, fiche 1, Anglais, - folliculo%20stellate%20derived%20growth%20factor

Record number: 1, Textual support number: 1 OBS

Tiré de: Proceedings of the National Academy of Sciences of USA, 86 (19), 1989, 7311-7315.  3, fiche 1, Anglais, - folliculo%20stellate%20derived%20growth%20factor

Fiche 1,  Justifications,  Français

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La vasculotropine est le dernier facteur angiogénique découvert. Il a été isolé à partir de cellules folliculaires de l'hypophyse de bœuf ou à partir du milieu conditionné de la lignée cellulaire hypophysaire AtT-20 (J. Plouet, communication personnelle). Il porte également le nom de folliculo stellate-derived growth factor (FSdGF).  1, fiche 1, Français, - vasculotropine

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Due to the drop in pressure that occurs in high-pressure liquid chromatography(HPLC) as the solvent passes from the column to the detector, any dissolved gases(most commonly air) are partially released as bubbles within the detector, producing anomalous and misleading results. To avoid this problem, solvents are degassed before use, either by a nitrogen purge or by heating and stirring under vacuum.  2, fiche 2, Anglais, - degassing

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The process of solute movement into and out of the stationary phase or mobile phase. It is represented by the C term of the van Deemter equation and is referred to as the mass transfer term. The faster the process of mass transfer, the better the efficiency of the column. In HPLC, mass transfer is the most important factor affecting column efficiency. It is increased by the use of small particle packings, thin layers of stationary phase, low viscosity mobile phases, and high temperatures.  3, fiche 3, Anglais, - mass%20transfer%20term

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The initial solubilization of yolk platelets demonstrated that the lipid was present in the soluble fraction containing lipovitellin and phosvitin. These two proteins have been resolved and the lipovitellin fraction was shown to exhibit the absorption maximum at 375 nm that characterizes DCD [dorsalizing cytoplasmic determinant]. The lipovitellin was purified and its total lipids extracted and separated on HPLC [high-performance liquid chromatography]. A lipid was found that has identical elution properties from a C18 reverse HPLC column and mass as DCD. Therefore, lipovitellin, a zinc protein known to contain 17% lipid by weight, is the oocyte storage protein for DCD.  2, fiche 4, Anglais, - lipovitellin

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The use of HPLC [high performance liquid chromatography] to determine the saturate content of heavy petroleum products.... The refractive index response factors for several pure saturate fractions isolated from heavy petroleum products were determined using a µ-Bondapak aminopropylmethylsilyl bonded amorphous silica HPLC column.  2, fiche 5, Anglais, - aminopropylmethylsilyl%20bonded%20amorphous%20silica

Record number: 5, Textual support number: 2 CONT

Column - Water carbohydrate analysis [trademark] partition column. Aminopropylmethylsilyl bonded amorphous silica, 300 mm x 3.9 mm (ID) or equivalent.  3, fiche 5, Anglais, - aminopropylmethylsilyl%20bonded%20amorphous%20silica

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A chromatographic technology used to separate and quantitate mixtures of substances in solution.  5, fiche 6, Anglais, - high%2Dperformance%20liquid%20chromatography

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The sample is injected into a moving stream of solvent, which flows through a column and detector. The passage through the column separates the sample compounds by one of four different methods: adsorption, partition, ion exchange, or size exclusion. The solvent is pumped through the system at a controlled pressure, which can be varied between 500 and 5000 psi. With the particular column and elution method used for the assay, the elution times relative to the internal standard characterize the compounds and are used to identify them. The peak areas relative to the internal standard are proportional to their concentration in the sample and are used to quantitate them.  6, fiche 6, Anglais, - high%2Dperformance%20liquid%20chromatography

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A special form of liquid chromatography, high-performance liquid chromatography(HPLC), has become especially important for the separation of complex mixtures of nonvolatile materials. Essentially, it involves the use of very small particle size in column packing, with high pressure to increase the rate of flow and shorten the separation time to a few minutes.  7, fiche 6, Anglais, - high%2Dperformance%20liquid%20chromatography

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One of the most significant advances in chromatography in recent years has been the introduction of high performance liquid chromatography. Originally intended for the separation of small organic molecules that were soluble in non-aqueous solvents, the technique rapidly developed into a form suitable for the separation of larger, biological compounds soluble in aqueous solvents. In recent years, following the development of macroporous supports, the technique has become applicable to molecules as large as proteins and nucleic acids.  8, fiche 6, Anglais, - high%2Dperformance%20liquid%20chromatography

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The term high pressure liquid chromatography (now obsolete) was used in the early stages of development of the technique since the quality of the columns was such that high pressures were needed to force the liquid through.  4, fiche 6, Anglais, - high%2Dperformance%20liquid%20chromatography

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Technique qui permet d'obtenir une séparation de mélanges complexes selon le même principe que la chromatographie liquide classique (échange d'ions, tamisage moléculaire, ou interaction hydrophobe) mais en des temps très courts grâce à l'emploi de hautes pressions.  12, fiche 6, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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Les avantages de l'HPLC [«high-performance liquid chromatography»] que sont la rapidité et la possibilité d'automatiser totalement l'analyse et le traitement des données (mise en mémoire et traitement informatique des courbes chromatographiques), sont susceptibles de conduire cette technique à remplacer, au moins dans certaines applications, l'électrophorèse classique actuelle.  12, fiche 6, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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La chromatographie liquide à haute performance (HPLC) est une méthode récente. L'évolution technologique a permis de réaliser des résines sphériques de taille allant jusqu'à 5 microns, ce qui leur donne des propriétés mécaniques telles qu'elles supportent de forts débits ainsi que la haute contre-pression qui en résulte. On réalise ainsi des séparations beaucoup plus rapides avec une meilleure résolution. L'appareil est constitué d'une colonne en acier inoxydable d'une longueur de l'ordre de 200 mm et de diamètre intérieur de 4 mm, remplie de particules de taille inférieure à 20 mm; cette colonne est précédée d'une pompe à haute pression, permettant d'injecter le solvant sous une pression de plusieurs dizaines de bars. Des microvannes assurent l'introduction de quelques microlitres de solution à analyser, en tête de colonne.  13, fiche 6, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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Chromatographie liquide à haute performance multidimensionnelle.  14, fiche 6, Français, - chromatographie%20liquide%20%C3%A0%20haute%20performance

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CLAR es una técnica analítica en la que una fase móvil líquida transporta una muestra a través de una columna que contiene una fase líquida estacionaria. La interacción de la muestra con la fase estacionaria retiene de modo selectivo los compuestos individuales y permite la separación de los componentes de la muestra [...]  2, fiche 6, Espagnol, - cromatograf%C3%ADa%20l%C3%ADquida%20de%20alta%20presi%C3%B3n

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Technique in which the mobile phase(fluid) is maintained at temperatures somewhat above its critical point. The mobile phases used in SFC are gases such as freon, ethylene, or carbon dioxide. It has superior solution properties and enhances the chromatographic separation of higher molecular weight compounds. The column packings used in SFC are the same as those used in HPLC.  2, fiche 7, Anglais, - supercritical%20fluid%20chromatography

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