TERMIUM Plus^®

Fiche 1,  Justifications,  Anglais

Record number: 1, Textual support number: 1 CONT

Frozen cells (about 20 g wet weight) harvested from about 150 liter culture with sodium thiosulfate were suspended in 270 ml of 10 mM Tris-HCl buffer, pH 8.0, and then disrupted in a French pressure cell (1,100 kg/cm2). All subsequent steps were performed under aerobic conditions at room temperature. Unbroken cells were removed by centrifugation at 10,000 x g for 15 min. The resulting supernatant was used as a cell-free extract.  3, fiche 1, Anglais, - cell%20free%20extract

Fiche 1,  Justifications,  Français

Record number: 1, Textual support number: 1 DEF

Broyat cellulaire plus ou moins purifié capable d'assurer certaines fonctions enzymatiques du vivant in vitro.  2, fiche 1, Français, - extrait%20acellulaire

Fiche 1,  Justifications,  Espagnol