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Fiche 1,  Justifications,  Anglais

Record number: 1, Textual support number: 1 DEF

A quantitative assay that counts antibody-producing cells. Lymphocytes sensitized against sheep erythrocytes (SRBCs) are placed in agar with SRBCs leaving a clear circular plaque around each cell that produced antibody against SRBC. The plaques are counted and reported as the number of plaque-forming cells (PFCs).  1, fiche 1, Anglais, - hemolytic%20plaque%20assay

Fiche 1,  Justifications,  Français

Record number: 1, Textual support number: 1 CONT

La technique des plages d'hémolyse consiste à introduire des cellules lymphoïdes et une population dense d'érythrocytes xénogéniques dans un milieu gélifié. Certaines cellules lymphoïdes libèrent des anticorps hémolytiques qui diffusent autour d'elles et entraînent la lyse des hématies de leur voisinage, en formant une plage de lyse (on parle en anglais de «plaque-forming cells» ou «PFC») quand on ajoute du complément. Les plages sont visibles et sont comptées avec une loupe.  1, fiche 1, Français, - technique%20des%20plages%20d%27h%C3%A9molyse

Fiche 1,  Justifications,  Espagnol

Fiche 2,  Justifications,  Anglais

Record number: 2, Textual support number: 1 DEF

A method used to detect and enumerate antibody producing cells. Cells obtained from, e.g. the spleen of an animal that has been immunized against sheep erythrocytes, are mixed into molten Agarose containing sheep erythrocytes. The agarose is poured into a Petri dish, allowed to set and then incubated at 37 ° C. Later, complement is poured into the dish, and any cells that have secreted antibody are revealed by the appearance of a zone of haemolysis round each of them. The agarose layer can be dried and stained so that the cells may be identified. The technique described reveals IgM secreting cells forming direct plaques. By the addition of an anti-IgG serum before the complement, indirect plaques due to IgG secreting cells can be revealed. Cells producing antibodies to antigens other than erythrocytes can be detected by coating the rythrocytes with the antigen as in passive haemolysis.  1, fiche 2, Anglais, - balmolytic%20plaque%20test

Fiche 2,  Justifications,  Français

Record number: 2, Textual support number: 1 CONT

La méthode de numération par la technique des plages de lyse est utilisée dans certains laboratoires [...] pour le titrage de suspensions de cultures pures.  2, fiche 2, Français, - technique%20des%20plages%20de%20lyse

Fiche 2,  Justifications,  Espagnol