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Fiche 1,  Justifications,  Anglais

Record number: 1, Textual support number: 1 CONT

DNase Agar. DM132 A medium for the presumptive identification of pathogenic staphylococci by demonstration of DNase production.  6, fiche 1, Anglais, - deoxyribonucleic%20acid%20agar

Record number: 1, Textual support number: 2 CONT

Deoxyribonuclease (DNAse) agar actually has DNA in it. Deoxyribonuclease is an exoenzyme excreted from the cell which will break the DNA down into smaller molecules. There are 2 kinds of DNAse agar that can be used. 1) The first medium has no indicator, but is does require HCl (hydrochloric acid) reagent to be added after growth has occurred on the plate. Without DNAse enzyme, the HCl precipitates out the undestroyed DNA. The rest of the agar with the remaining DNA, which binds to HCl will have turned cloudy. Around the growth area, if the bacterium has made DNAse and destroyed the DNA, there will be clear zones seen. 2) DNAse agar with the indicator methyl green can be used. In this medium, when the DNA in the medium is hydrolyzed by the bacterial DNAse enzyme, the pieces of cut DNA release from binding to methyl green, and you will see a yellowish/orange zone around the growth area. OBJECTIVE: To identify the presence of the DNAse enzyme.  3, fiche 1, Anglais, - deoxyribonucleic%20acid%20agar

Record number: 1, Textual support number: 1 PHR

DNase agar with (w/) toluidine blue, methyl green.  7, fiche 1, Anglais, - deoxyribonucleic%20acid%20agar

Fiche 1,  Justifications,  Français

Record number: 1, Textual support number: 1 OBS

La gélose de test DNase est un milieu de culture pour détecter la production de désoxyribonucléase par des bactéries, en particulier les staphylocoques.  4, fiche 1, Français, - g%C3%A9lose%20%C3%A0%20l%26rsquo%3Bacide%20d%C3%A9soxyribonucl%C3%A9ique

Record number: 1, Textual support number: 2 OBS

La désoxyribonucléase (ADNase [ou DNase]) est une enzyme qui catalyse les acides désoxyribonucléiques (ADN).  1, fiche 1, Français, - g%C3%A9lose%20%C3%A0%20l%26rsquo%3Bacide%20d%C3%A9soxyribonucl%C3%A9ique

Record number: 1, Textual support number: 1 PHR

Gélose à ADN au bleu de toluidine, au vert de méthyle.  1, fiche 1, Français, - g%C3%A9lose%20%C3%A0%20l%26rsquo%3Bacide%20d%C3%A9soxyribonucl%C3%A9ique

Record number: 1, Textual support number: 2 PHR

Gélose DNase au bleu de toluidine, au vert de méthyle.  1, fiche 1, Français, - g%C3%A9lose%20%C3%A0%20l%26rsquo%3Bacide%20d%C3%A9soxyribonucl%C3%A9ique

Fiche 1,  Justifications,  Espagnol

Fiche 2,  Justifications,  Anglais

Record number: 2, Textual support number: 1 DEF

A method of separating molecules of different molecular weight or of the same molecular weight but different configuration by passing them through a gel column under the influence of an applied electric field.  2, fiche 2, Anglais, - agarose%20gel%20electrophoresis

Record number: 2, Textual support number: 1 OBS

In general, higher molecular weight molecules pass through faster than lower molecular weight molecules, the migration rates of the molecules being inversely proportional to the logarithms of their molecular weights. Represented a breakthrough in rec DNA technology since it was the first accurate method of sizing DNA molecules.  2, fiche 2, Anglais, - agarose%20gel%20electrophoresis

Fiche 2,  Justifications,  Français

Record number: 2, Textual support number: 1 DEF

Méthode de fractionnement, qui opère une ségrégation des molécules d’ADN en fonction de la taille. Le support est constitué à l'aide d’une solution de gélose(agar-agar) à 1 p. 100 dans le tampon choisi. On la coule à chaud sur une lame de verre où elle se solidifie par refroidissement. Le pouvoir de résolution est identique à celui du papier. Mais le gel d’agarose purifiée présente l'avantage de ne pas produire d’interaction avec les protéines.  3, fiche 2, Français, - %C3%A9lectrophor%C3%A8se%20en%20gel%20d%26rsquo%3Bagarose

Fiche 2,  Justifications,  Espagnol

Record number: 2, Textual support number: 1 DEF

[Método] para analizar y caracterizar ácidos nucleicos de distintas procedencias.  1, fiche 2, Espagnol, - electroforesis%20en%20gel%20de%20agarosa

Record number: 2, Textual support number: 1 CONT

Los geles se comportan como un tamiz molecular y permiten separar moléculas cargadas en función de su tamaño y forma. Así, moléculas de DNA de diferente tamaño van a emigrar de forma distinta en una electroforesis en gel de agarosa. Además, si en dicha electroforesis se aplican marcadores de peso molecular (fragmentos de DNA de tamaño conocido) se puede calcular el tamaño aproximado del DNA en estudio.  1, fiche 2, Espagnol, - electroforesis%20en%20gel%20de%20agarosa