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The Government of Canada’s terminology and linguistic data bank.
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Record 1 - internal organization data 2012-03-06
Record 1, English
Record 1, Subject field(s)
- Genetics
Record 1, Main entry term, English
- primer
1, record 1, English, primer
correct, noun
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
A short sequence of single-stranded DNA [deoxyribonucleic acid] or RNA [ribonucleic acid] that is paired with one complementary strand of DNA and provides a free 3'-OH end at which a DNA polymerase starts synthesis of a deoxyribonucleotide chain. 2, record 1, English, - primer
Record number: 1, Textual support number: 1 CONT
The polymerase chain reaction (PCR) is a method of copying a DNA or RNA strand thousands of times, in a matter of minutes, by using the natural ability of an enzyme called polymerase to copy DNA. ... Scientists must know ... what DNA sequences flank or frame either ends of the desired portion. Complementary copies of the DNA sequences that flank the ends are created by using special enzymes; they are called primers. They identify the start and end of the copying process. ... Since primers are complementary to the beginning and ending regions of the desired portion of the DNA sequence, they will bond to those areas and act as a building block for the copying process to begin and end. 3, record 1, English, - primer
Record 1, French
Record 1, Domaine(s)
- Génétique
Record 1, Main entry term, French
- amorce
1, record 1, French, amorce
correct, feminine noun, standardized
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Oligonucléotide qui, hybridé avec une matrice d'acide nucléique, permet à une polymérase d'initier la synthèse du brin complémentaire. [Définition normalisée par l’AFNOR et reproduite avec son autorisation.] 2, record 1, French, - amorce
Record number: 1, Textual support number: 1 CONT
Un cycle d'amplification comprend trois étapes : une phase de dénaturation thermique [...] de l'ADN [acide désoxyribonucléique] qui sépare les deux chaînes complémentaires; une phase d'appariement de ces chaînes avec de courts segments d'ADN (oligonucléotides synthétiques appelés amorces) présents en grande quantité et dont la séquence nucléotidique est complémentaire des extrémités de la cible; enfin une phase d'élongation par l'ADN polymérase qui ajoute, à l'extrémité de l'amorce appariée à la cible, les unités de monomères complémentaires à ceux de la séquence cible. 3, record 1, French, - amorce
Record number: 1, Textual support number: 2 CONT
Les amorces ont deux fonctions, le repérage de l'ADN recherché, puis l'amorçage de la réplication de cet ADN par un ADN polymérase. 4, record 1, French, - amorce
Record number: 1, Textual support number: 1 OBS
amorce : terme normalisé par l’AFNOR et publié au Journal officiel de la République française le 22 septembre 2000. 5, record 1, French, - amorce
Record 1, Spanish
Record 1, Campo(s) temático(s)
- Genética
Record 1, Main entry term, Spanish
- cebador
1, record 1, Spanish, cebador
correct, masculine noun
Record 1, Abbreviations, Spanish
Record 1, Synonyms, Spanish
Record 1, Textual support, Spanish
Record number: 1, Textual support number: 1 DEF
Oligonucleótido de tamaño pequeño que, al hibridar con un molde de ADN de un hebra, le proporciona una estructura bicatenaria a partir de la cual, la ADN polimerasa sintetizará una nueva hebra de ADN para producir una molécula dúplex. 1, record 1, Spanish, - cebador
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