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Record 1 1997-05-20

English

Subject field(s)
  • Genetics
  • Molecular Biology
CONT

After hydrolysis of the template RNA, the cDNA is detected by a sandwich hybridization assay (Enzyme-Linked Oligonucleotide Sorbent Assay, or ELOSA). The sandwich consists of a biotinylated capture probe, the cDNA, and an HRP-conjugated detector probe. The sandwich is bound via the biotin to a streptavidin-coated microplate well, and is detected by adding a substrate for HRP. Although all steps may be performed manually, the ELOSA system is fully compatible with automated ELISA (Enzyme-Linked ImmunoSorbent Assay) plate washers and readers.

French

Domaine(s)
  • Génétique
  • Biologie moléculaire
CONT

L'ARN ribosomal cible est reconnu par deux sondes spécifiques : une sonde de capture qui permet la fixation du duplex ARN/ADN sur un «stick» et une sonde de détection enzymatique pour mettre en évidence ce duplex, par addition d'un substrat chromogène.

OBS

Détection des bactéries pathogènes alimentaires.

Spanish

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