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CONFOCAL MICROSCOPY [1 record]
Record 1 - internal organization data 2017-01-04
Record 1, English
Record 1, Subject field(s)
- Optics
- The Skin
Record 1, Main entry term, English
- confocal microscopy
1, record 1, English, confocal%20microscopy
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Confocal microscopy. Unlike "normal" microscopy, only a spot of a minimum size in the specimen is illuminated in confocal microscopy. To enable an image to be formed from this, the specimen must be scanned. The image of the spot is directed through a pinhole stop in an intermediate image plane. As a result, only light from the focal plane can reach the detector (a photomultiplier). All other (out-of-focus) planes are blocked out. This results in an "optical section". The images are stored electronically and displayed on a monitor. A series of optical sections can be recorded by moving a motor a slight distance along the z-axis each time an image has been recorded, after which the next image is then also recorded. Such a z-series permits the electronic reconstruction of the three-dimensional structure using suitable computer programs. The procedure, which is restricted to incident-light techniques, has completely revolutionized fluorescence microscopy in biology in particular. 2, record 1, English, - confocal%20microscopy
Record 1, French
Record 1, Domaine(s)
- Optique
- Appareil cutané
Record 1, Main entry term, French
- microscopie confocale
1, record 1, French, microscopie%20confocale
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La technique de microscopie confocale mise au point [...] pour l'imagerie cutanée in vivo est issue d'une adaptation du Tandem Scanning Microscope (TSM), un microscope à balayage optique en temps réel. Le principe confocal utilise la lumière blanche en mode réflexion et un disque rotatif pour donner une image nette dans la profondeur d'un tissu, en éliminant de l'image toutes les informations situées au-dessus et en dessous du plan focal. 2, record 1, French, - microscopie%20confocale
Record number: 1, Textual support number: 2 CONT
Un des aspects les plus intéressants de la microscopie confocale, outre son caractère non destructif, est sa rapidité de mise en œuvre; elle comble un fossé important entre la microscopie optique conventionnelle et la microscopie électronique. La localisation de molécules spécifiques, dans le contexte cellulaire, apporte d'importantes contributions à l'étude fonctionnelle des systèmes biologiques à différents niveaux d'organisation : subcellulaire, cellulaire, tissulaire ou organisme entier. L'utilisation de traceurs fluorescents très spécifiques permet de localiser in situ avec une résolution spatiale de l'ordre de 400 - 500 nanomètres, des composants macro-moléculaires tels que acides nucléiques et protéines, mais aussi de petites molécules telles que des peptides, lipides et même des ions. 2, record 1, French, - microscopie%20confocale
Record number: 1, Textual support number: 1 OBS
La microscopie confocale permet la localisation in situ d'une sonde fluorescente dans des cellules d'un spécimen d'une épaisseur de 10 mm à 50 mm selon certains auteurs (5 mm à 30 mm plus raisonnablement), sans sectionnement mécanique préalable. Son concurrent est le laser krypton (longueur d'onde) qui permet une excitation des fluorochromes situés dans le Vert (Texas-Red, Cyanin Blue). 3, record 1, French, - microscopie%20confocale
Record 1, Spanish
Record 1, Textual support, Spanish
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