TERMIUM Plus®

The Government of Canada’s terminology and linguistic data bank.

AFFINITY CHROMATOGRAPHY [1 record]

Filter results by subject field Alphabetical list of terms

Filter results by subject field

Alphabetical list of terms

Record 1 2011-05-17

English

Subject field(s)
  • Analytical Chemistry
  • Biochemistry
  • Biotechnology
DEF

A procedure which uses a gel matrix combined with an enzyme or nucleic acid to obtain purification of biomolecules.

DEF

A technique of molecular separation in which molecules are attached to an insoluble (e.g., sepharose) matrix. Only those molecules which show affinity to the bound molecule (e.g., an antibody for its antigen) are retained. These trapped molecules can be subsequently eluted.

Key term(s)
  • affinity purification

French

Domaine(s)
  • Chimie analytique
  • Biochimie
  • Biotechnologie
DEF

Technique permettant la séparation de substances, [...] qui utilise la fixation spécifique d'une protéine sur un ligand lui-même fixé par liaison covalente à un support microscopique.

CONT

[...] la chromatographie d'affinité permet de purifier une protéine enzymatique par exemple. La méthode consiste à attacher de façon covalente sur un support solide un analogue stérique du substrat de l'enzyme (inhibiteur compétitif ou substrat modifié) en vue de former avec l'enzyme un complexe du type enzyme-substrat [...]. L'enzyme sera donc retardé lors du passage sur la colonne par rapport aux autres protéines qui, ne reconnaissant pas l'analogue, sortiront de la colonne plus rapidement que l'enzyme.

Spanish

Campo(s) temático(s)
  • Química analítica
  • Bioquímica
  • Biotecnología
Save record 1

Copyright notice for the TERMIUM Plus® data bank

© Public Services and Procurement Canada, 2024
TERMIUM Plus®, the Government of Canada's terminology and linguistic data bank
A product of the Translation Bureau

Display options

Change the order of display of the official languages of Canada
Option to display the non-official languages (Spanish or Portuguese)
Display definitions, contexts, etc., on records

Features

Language Portal of Canada

Access a collection of Canadian resources on all aspects of English and French, including quizzes.

Writing tools

The Language Portal’s writing tools have a new look! Easy to consult, they give you access to a wealth of information that will help you write better in English and French.

Glossaries and vocabularies

Access Translation Bureau glossaries and vocabularies.

Date Modified: