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AFFINITY ELUTION [1 record]
Record 1 - internal organization data 2011-08-09
Record 1, English
Record 1, Subject field(s)
- Biological Sciences
Record 1, Main entry term, English
- affinity elution
1, record 1, English, affinity%20elution
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 DEF
An affinity purification technique based on 'non-specific' adsorption to an ion exchanger. Cation exchangers appear to be particularly useful for this purpose. Purification specificity is achieved using gradient elution with substrate. 1, record 1, English, - affinity%20elution
Record number: 1, Textual support number: 1 CONT
Affinity elution is complementary to affinity chromatography in that in the former technique the specificity of interaction is at the stage of desorption from the chromatographic support material, whereas in the latter the specificity occurs at the stage of adsorption. In affinity elution the enzyme of interest (along with others) is adsorbed onto an ion exchanger ... and then is eluted specifically by the appropriate substrate. 2, record 1, English, - affinity%20elution
Record 1, French
Record 1, Domaine(s)
- Sciences biologiques
Record 1, Main entry term, French
- élution par un substrat
1, record 1, French, %C3%A9lution%20par%20un%20substrat
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
Record 1, Textual support, French
Record number: 1, Textual support number: 1 DEF
Technique de séparation des protéines qui est l'inverse de la chromatographie d'affinité : l'enzyme est adsorbée sur un échangeur d'ion et éluée par une solution de substrat, d'inhibiteur compétitif ou d'effecteur allostérique. Exemple : la ribonucléase, adsorbée sur phosphocellulose, est éluée par une solution de RNA. 2, record 1, French, - %C3%A9lution%20par%20un%20substrat
Record 1, Spanish
Record 1, Textual support, Spanish
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