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CROMOSOMA ARTIFICIAL BACTERIANO [1 record]

Record 1 2007-08-08

English

Subject field(s)
  • Genetics
CONT

The most important aspect of our cloning vectors is that they are based on the E. coli F-factor replicon. It allows for strict copy number control of the clones so that they are stably maintained at 1-2 copies per cell. The stability of the cloned DNA during propagation in E. coli host is substantially higher in lower-copy number vectors than in multi-copy counterparts. ... The stabilizing effect of BAC and cosmid vectors is notable especially for certain genomic DNA that are normally unstable in high copy number vectors.

French

Domaine(s)
  • Génétique
DEF

Fragment d'ADN cloné dans une souche d'E. coli contenant le facteur F.

OBS

L'avantage [du facteur F] est sa grande efficacité de clonage, la stabilité de l'insert et la facilité de manipulation et de préparation de l'ADN cloné qui représente une source facile à produire.

Spanish

Campo(s) temático(s)
  • Genética
DEF

Vector de clonación generado en el laboratorio que permite insertar segmentos largos de ADN [...] provenientes de otras especies en el genoma de bacterias.

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