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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

… the use of a holographic beamsplitter allows additional control over the electron beam, such as removing the spherical aberration or introducing phase vortices that can be used to measure magnetic fields.  2, record 1, English, - holographic%20beamsplitter

Record 1,  Textual support,  French

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

The optical filter set. A set of optical filters for selective excitation and detection of fluorescence typically consists of a minimum of three components : an excitation filter, a dichroic beamsplitter("dichroic mirror") and an emission filter("barrier filter")...  1, record 2, English, - optical%20filter%20set

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Un microscope [à] épifluorescence est pourvu de plusieurs jeux de filtres correspondant aux fluorochromes les plus habituellement utilisés. Chaque jeu de filtres est constitué d'un filtre d'excitation, d'un miroir dichroïque et d'un filtre d'émission.  5, record 2, French, - jeu%20de%20filtres%20optiques

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 OBS

The Molecular Dynamics Sarastro 2000 has a simple light path that makes it ideal for conversion to a 2-photon scope. We retained the ability to use the microscope in the 1-photon, confocal mode by replacing mirror 2 with a beamsplitter that passes IR from the Mira 900 or reflects visible excitation from the on-board 25 mW argon-ion laser. Mirror 3 and the two scanning mirrors were replaced with broad-band mirrors for better IR throughput. We installed a plate that blocks the IR laser beam whenever the lid is opened to change filters, for safety. That is it--No more modifications necessary! To use in the 2-Photon mode, the on-board laser is turned off, the Mira 900 IR laser is turned on, the primary dichroic wheel is rotated to a 680 SP(Chroma) mirror, and the excitation filter and aperture wheels are rotated to the "open" settings. To improve rejection of excitation light, a barrier filter(680 SP, Chroma) is used in the detector housing.  3, record 3, English, - 2%2Dphoton%20imaging

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 OBS

Deux photons peuvent combiner leurs énergies pour provoquer le passage vers un état excité plus élevé du fluorochrome [Gu and Sheppard 1995], (références, exemple de transformation d'un confocal pour (l'imagerie 2-photons): deux photons situés dans le Rouge peuvent exciter un fluorochrome dont la gamme d'onde correspond aux UV. Les dommages opérés généralement par les rayons UV sont donc évités. En outre, cette technique minimise le phénomène d'évanescence (photobleaching) de la sonde et autorise une meilleure pénétration dans le tissu. Enfin, les dommages du laser sont très réduits dans le cas de l'imagerie multi-photons en raison d'une faible émission du signal laser au niveau des plans non focaux.  1, record 3, French, - imagerie%20multi%2Dphotons

Record number: 3, Textual support number: 2 OBS

Caractéristique du microscope confocal.  2, record 3, French, - imagerie%20multi%2Dphotons

Record 3,  Textual support,  Spanish