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BRIGHT FIELD ILLUMINATION [1 record]
Record 1 - internal organization data 2015-09-09
Record 1, English
Record 1, Subject field(s)
- Optics
- Biophysics
- Chemistry
Record 1, Main entry term, English
- bright-field optical microscopy
1, record 1, English, bright%2Dfield%20optical%20microscopy
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
- bright field optical microscopy 1, record 1, English, bright%20field%20optical%20microscopy
correct
- bright-field microscopy 2, record 1, English, bright%2Dfield%20microscopy
correct
- brightfield microscopy 2, record 1, English, brightfield%20microscopy
correct
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Brightfield microscopy is the most elementary form of microscope illumination techniques... The name "brightfield" is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.... In brightfield microscopy a specimen is placed on the stage of the microscope and incandescent light from the microscope's light source is aimed at a lens beneath the specimen.... light passes through the specimen and then is collected by an objective lens situated in a turret above the stage.... This technique can be used to view fixed specimens or live cells. Since many organic specimens are transparent or opaque, staining is required to cause the contrast that allows them to be visible under the microscope. 2, record 1, English, - bright%2Dfield%20optical%20microscopy
Record 1, Key term(s)
- brightfield optical microscopy
- brightfield microscopy
Record 1, French
Record 1, Domaine(s)
- Optique
- Biophysique
- Chimie
Record 1, Main entry term, French
- microscopie optique en champ clair
1, record 1, French, microscopie%20optique%20en%20champ%20clair
correct, feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- microscopie en champ clair 1, record 1, French, microscopie%20en%20champ%20clair
correct, feminine noun
- microscopie optique à fond clair 1, record 1, French, microscopie%20optique%20%C3%A0%20fond%20clair
correct, feminine noun
- microscopie à fond clair 2, record 1, French, microscopie%20%C3%A0%20fond%20clair
correct, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La microscopie optique en champ clair (ou «à fond clair») est la plus simple et la plus ancienne des techniques de microscopie. [...] L'illumination se fait par transmission de lumière blanche, c'est-à-dire que l'échantillon est illuminé par dessous et observé par dessus. Les limitations de cette technique sont principalement un faible contraste de la plupart des échantillons biologiques et une résolution faible due au flou créé par la matière hors du plan focal. En contrepartie, la technique est simple et l'échantillon ne nécessite qu'une préparation minime. 1, record 1, French, - microscopie%20optique%20en%20champ%20clair
Record number: 1, Textual support number: 1 OBS
La microscopie à fond clair permet d'observer des préparations colorées (sans coloration, on ne voit rien). 3, record 1, French, - microscopie%20optique%20en%20champ%20clair
Record 1, Spanish
Record 1, Textual support, Spanish
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