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CROSSLINKING REAGENT [3 records]

Record 1 2011-03-14

English

Subject field(s)
  • Chemical Elements and Compounds
Universal entry(ies)
Zn[OC(CH3):CHCO(CH3)]4
formula, see observation
17501-44-9
CAS number
DEF

A colorless, crystalline tetrachelate;... soluble in pyridine, acetone, benzene, and other organic solvents having some polarity; slightly soluble in water.... Use :Crosslinking agent for polyol, polyester, and polylkyoxy resins; lubricant and grease additive; reagent; catalyst.

OBS

Chemical formula: Zn[OC(CH3):CHCO(CH3)]4

French

Domaine(s)
  • Éléments et composés chimiques
Entrée(s) universelle(s)
Zn[OC(CH3):CHCO(CH3)]4
formula, see observation
17501-44-9
CAS number
OBS

Formule chimique : Zn[OC(CH3):CHCO(CH3)]4

Spanish

Save record 1

Record 2 1989-12-19

English

Subject field(s)
  • Biological Sciences
CONT

In many cases the presence of an insoluble or even soluble carrier matrix may become an insuperable obstacle for the enzyme to perform its function in the organism. In such cases investigators have used bifunctional reagents to stabilize enzymes by intramolecular crosslinking. As a rule these attempts have had only limited success, based on trials with many enzymes.... The matter is complicated by the fact that the bifunctional reagent can interact with the enzyme in three different ways : one point modification, intermolecular linking, and intramolecular crosslinking. The observed stabilization effect can be explained easily by a simple modification to the enzyme or by changes in the microenvironment as a result of the modification.

French

Domaine(s)
  • Sciences biologiques
CONT

L'insolubilisation des enzymes requiert (...) une "greffe" dans des conditions douces, entre R-COOH ou R-NH2 de la protéine et certains groupements réactifs du support solide. (...) Les agents du couplage enzyme-support sont en bonne règle des agents bifonctionnels, actifs dans des conditions douces (glutaraldéhyde, carbodiimides, etc.). Souvent, la liaison n'est pas effectuée en une seule étape, mais le support est d'abord activé, puis la protéine lui est fixée.

Spanish

Save record 2

Record 3 1989-12-13

English

Subject field(s)
  • Biological Sciences
CONT

The most suitable gel material requires both a relatively high concentration of the monomer(acrylamide) to give mechanical rigidity and a high concentration of crosslinking reagent(N, N. methylenebisacrylamide) to achieve the highest possible yield of immobilized enzyme activity.... The trapping efficiency of polyacrylamide gels of varying composition was examined by several investigators. Degani and Miron observed that the maximum yield of activity trapping(56%) occurred with cholinesterase at a crosslinking concentration of 5% and with a total and constant monomer concentration of 15%.

French

Domaine(s)
  • Sciences biologiques

Spanish

Save record 3

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