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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Strand displacement amplification(SDA) [is] a fully automated method that amplifies target nucleic acid without the use of a thermocycler. A double-strand DNA fragment is created and becomes the target for exponential amplification.  2, record 1, English, - strand%20displacement%20amplification

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 OBS

Cette réaction est isotherme; la déshybridation est entretenue par une enzyme de restriction à la place de l'action de la chaleur [...]  1, record 1, French, - amplification%20par%20d%C3%A9placement%20de%20brin

Record 1,  Textual support,  Spanish

Record number: 1, Textual support number: 1 CONT

Métodos moleculares como la Reacción en Cadena de la Polimerasa ( PCR ), Reacción en Cadena de la Ligasa (LCR), Amplificación Basada en Secuencias de Ácidos Nucleicos (NASBA), y Amplificación por Desplazamiento de Cadena (SDA) se comercializan en forma de kits y es introducido en el diagnóstico de rutina lo cual brinda una mayor garantía de obtener buenos resultados.  1, record 1, Spanish, - amplificaci%C3%B3n%20por%20desplazamiento%20de%20cadenas

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.  4, record 2, English, - fluorescence%20in%20situ%20hybridization

Record number: 2, Textual support number: 1 CONT

Until recently, even the best chromosomal maps could be used to locate a DNA [deoxyribonucleic acid] fragment only to a region of about 10 Mb, the size of a typical band seen on a chromosome. Improvements in fluorescence in situ hybridization(FISH) methods allow orientation of DNA sequences that lie as close as 2 to 5 Mb.  2, record 2, English, - fluorescence%20in%20situ%20hybridization

Record number: 2, Textual support number: 2 CONT

Fluorescent in situ hybridization on interphase nuclei prepared from normal male fibroblast cultures gave a distance between D9S12 and D9S180 of about one Mb.  3, record 2, English, - fluorescence%20in%20situ%20hybridization

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Le développement des techniques d'hybridation in situ en fluorescence et la mise au point de systèmes d'imagerie dont les performances répondent aux exigences des cytogénéticiens permettent aujourd'hui la mise en œuvre de méthodes analytiques puissantes, clés de l'investigation efficace du génome et de ses anomalies.  2, record 2, French, - hybridation%20in%20situ%20en%20fluorescence

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

[Técnica] que utiliza sondas de ADN [ácido desoxirribonucleico] marcadas con fluorescencia para detectar o confirmar anomalías génicas o cromosómicas que generalmente están más allá del poder de resolución de la citogenética de rutina.  1, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 2, Textual support number: 1 CONT

Fluorescence in situ hybridization (FISH) o hibridación fluorescente in situ es una técnica que combina citogenética clásica con biología molecular. Con esta asociación se logra obtener la información genética manteniendo la estructura de las células o tejidos estudiados. [...] FISH es una herramienta importante en investigación y se incrementa su uso en diagnóstico humano. [...] ejemplos de su aplicación [...]: genética pre y postnatal; diagnóstico, seguimiento y/o pronóstico en cáncer; determinaciones de anomalías en embriones de preimplantación; microbiología; radiobiología; medio ambiente y evolución.  2, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record number: 2, Textual support number: 1 OBS

FISH por su sigla en inglés.  3, record 2, Spanish, - hibridaci%C3%B3n%20in%20situ%20fluorescente

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

During the study, we found that the Taq DNA polymerase used for PCR [polymerase chain reaction] adds on a single extra base(usually an A) at the end of a large fraction of the newly synthesized chains. These had to be removed by the Klenow fragment of DNA polymerase to insure restoration of the gene sequence.  3, record 3, English, - Taq%20polymerase

Record number: 3, Textual support number: 1 OBS

Used for polymerase chain reaction.  1, record 3, English, - Taq%20polymerase

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

ADN polymérase thermorésistante extraite de la bactérie Thermus aquaticus et utilisée par l'amplification élective du ADN in vitro [...] à température élevée (aux environs de 70°).  3, record 3, French, - Taq%20polym%C3%A9rase

Record number: 3, Textual support number: 1 OBS

Taq : Thermus aquaticus.  4, record 3, French, - Taq%20polym%C3%A9rase

Record 3,  Textual support,  Spanish

Record number: 3, Textual support number: 1 DEF

ADN polimerasa termoestable aislada de la bacteria termofílica Thermus aquaticus, muy utilizada en [reacción en cadena de la polimerasa].  1, record 3, Spanish, - polimerasa%20Taq

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 DEF

[A DNA segment located at the extremity of a chromosome, that possesses] special properties, among them a polarity that prevents their reunion with any fragment after a chromosome has been broken.  2, record 4, English, - telomere

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 DEF

Segment d'ADN situé à l'extrémité d'un chromosome, qui ne porte aucun gène et qui protège le chromosome contre les dégradations qui peuvent se produire à chaque réplication.  2, record 4, French, - t%C3%A9lom%C3%A8re

Record 4,  Textual support,  Spanish

Record number: 4, Textual support number: 1 DEF

Porción extrema de los brazos cromosómicos.  2, record 4, Spanish, - tel%C3%B3mero

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 CONT

A DNA fragment containing the untranslated leader and the proximal 2, 5 kb of the promoter directs expression of the GUS reporter gene in transgenic potatoes in a similar way as the agpS promoter, i. e. very strong expression in tuber and a more temperate expression in perivascular starch sheath, stomata guard cells, pollen and placenta. No expression at all could be detected in roots and in mesophyll cells. As in the case of agpS, another gene thus need to be expressed in these contexts to sustain amylosynthesis.  1, record 5, English, - amylosynthesis

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 OBS

Synthèse de l'amidon.  1, record 5, French, - amylosynth%C3%A8se

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 DEF

A technique based on polymerase chain reaction(PCR) amplification of DNA fragment using single primer with an arbitrary nucleotide sequence.  2, record 6, English, - random%20amplification%20of%20polymorphic%20DNA

Record number: 6, Textual support number: 1 PHR

Random amplification of polymorphic DNA analysis, assay, marker, method, technique.  2, record 6, English, - random%20amplification%20of%20polymorphic%20DNA

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 DEF

Technique de génotypage basée sur la PCR [réaction en chaîne de la polymérase] dans laquelle une matrice génomique est amplifiée par une seule amorce courte (généralement 10-mer [unité monomère]) choisie aléatoirement.  1, record 6, French, - amplification%20al%C3%A9atoire%20de%20l%27ADN%20polymorphe

Record number: 6, Textual support number: 1 PHR

Analyse, essai, marqueur, méthode, technique d'amplification aléatoire de l'ADN polymorphe.  2, record 6, French, - amplification%20al%C3%A9atoire%20de%20l%27ADN%20polymorphe

Record 6,  Textual support,  Spanish

Record number: 6, Textual support number: 1 DEF

Técnica de genotipado, basada en la reacción en cadena de la polimerasa, en la que se amplifica un molde genómico con un único cebador corto (10 nucleótidos) elegido al azar.  1, record 6, Spanish, - ADN%20polim%C3%B3rfico%20amplificado%20al%20azar

Record number: 6, Textual support number: 1 OBS

Los fragmentos obtenidos son el resultado de la amplificación de regiones de hasta 2 kpb [kilo pares de bases] de longitud, que se separan por electroforesis.  1, record 6, Spanish, - ADN%20polim%C3%B3rfico%20amplificado%20al%20azar

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 DEF

A DNA fragment on which research or analysis is performed.  2, record 7, English, - target%20DNA

Record number: 7, Textual support number: 1 CONT

A human chromosome is a DNA [desoxyribonucleic acid] molecular consisting of about 108 basepairs. A physical map is defined to be a piece of DNA that tells us the location of certain markers along the molecule. The markers are typically small, but precisely defined, sequences. To build physical maps, we begin by making several copies of the DNA molecule to be mapped, the so-called target DNA.  3, record 7, English, - target%20DNA

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 DEF

Fragments d'ADN désignés pour être l'objet d'analyses ou de recherches.  2, record 7, French, - ADN%20cible

Record number: 7, Textual support number: 1 CONT

Lors d'une analyse, on cherche à détecter des fragments de matériel génétique (ADN ou ARN cibles) contenus dans un échantillon biologique. L'ADN [acide désoxyribonucléique] à analyser peut être celui des cellules d'un patient ou bien celui d'un microorganisme infectieux.  3, record 7, French, - ADN%20cible

Record 7,  Textual support,  Spanish

Record 8,  Textual support,  English

Record number: 8, Textual support number: 1 DEF

A cloning vehicle which has cleavage sites for two or more restriction endonucleases, thus defining a fragment of DNA which can be removed and replaced by foreign DNA.  2, record 8, English, - replacement%20vector

Record number: 8, Textual support number: 1 CONT

Making more subtle mutations: the "knock in". Although it is often desirable to simply inactivate a gene to determine the importance of a null phenotype, in many cases it is more informative not to inactivate a gene, but rather to modify it so that its function is altered. Again, this is a task (which is the logical equivalent of site directed mutagenesis in a plasmid) that can be solved by homologous recombination. In this case a targeting vector is designed as a replacement vector so that additional genetic sequence are added into the genome. This technique is sometimes called a "knock-in".  2, record 8, English, - replacement%20vector

Record 8,  Textual support,  French

Record number: 8, Textual support number: 1 DEF

Véhicule de clonage comportant un ou plusieurs sites de coupure pour une enzyme de restriction donnée définissant ainsi un fragment d'ADN pouvant être éliminé et remplacé par de l'ADN étranger.  2, record 8, French, - vecteur%20de%20remplacement

Record number: 8, Textual support number: 1 CONT

Thomas et Capecchi ont au contraire muté le gène Hprt dans des cellules ES en utilisant deux types de construction déduits des mécanismes de recombinaison connus chez la levure : des vecteurs de remplacement, dont les séquences viennent remplacer les séquences homologues endogènes, et des vecteurs d'insertion, dont les séquences s'insèrent au milieu des séquences endogènes.  3, record 8, French, - vecteur%20de%20remplacement

Record 8,  Textual support,  Spanish

Record 9,  Textual support,  English

Record number: 9, Textual support number: 1 DEF

Site-directed mutagenesis technique based on the use of an oligodeoxynucleotide cassette(a synthetic double-stranded DNA fragment). The strategy involves the introduction of unique silent restriction sites designed to closely flank the target codon. Digestion with appropriate restriction enzymes produce a gap that can be filled with a duplex synthetic oligonucleotide cassette. The cassette is designed to restore the coding sequence in the gap and to introduce an altered target codon.  2, record 9, English, - cassette%20mutagenesis

Record 9,  Textual support,  French

Record number: 9, Textual support number: 1 DEF

Technique de mutagénèse dirigée in vitro qui consiste à créer dans le gène une "cassette" encadrée par deux sites d'enzyme de restriction. Il est ensuite aisé de remplacer cette cassette par une autre, constituée de deux brins d'ADN synthétique, pour introduire la mutation que l'on veut dans la zone de la cassette. Les deux sites de restriction seront souvent obtenus par mutagénèse dirigée.  2, record 9, French, - mutag%C3%A9n%C3%A8se%20par%20cassette

Record number: 9, Textual support number: 1 OBS

Cette méthode est très efficace, puisque l'on ramène la mutagénèse à un simple clonage, et se prête bien à l'exploration systématique de la séquence d'une petite protéine dont on aura éventuellement synthétisé tout le gène (cas du tPA), ou d'une zone définie d'une protéine plus grosse, comme les zones hypervariables des anticorps par exemple.  1, record 9, French, - mutag%C3%A9n%C3%A8se%20par%20cassette

Record 9,  Textual support,  Spanish

Record 10,  Textual support,  English

Record number: 10, Textual support number: 1 CONT

Short tandem repeat polymorphisms, also referred to as variable number of tandem repeats(VNTRs), are very important to scientists, because they can be used to identify individuals genetically. Marker systems are used to detect loci that respond to restriction enzyme cleavage by producing a fragment that can have many different lengths. This polymorphism is due to variation in the number of tandem repeats. VNTRs can be identified in various ways. Probes can be developed which will hybridize to complementary sequences after RFLP has been performed on a sample of genomic DNA(Southern blots). A second method involves the use of PCR. If the sequence surrounding the VNTR is known, PCR primers(oligonucleotides) can be designed to amplify the repeat region(s). The information obtained from these experiments is useful in the fields of forensics, population characterization, human gene mapping, identification of genes involved in disease, relationship testing, and identifying genes of interest such as those that encode proteins containing zinc finge.  3, record 10, English, - variable%20number%20of%20tandem%20repeats

Record 10,  Textual support,  French

Record number: 10, Textual support number: 1 CONT

Ces expertises sont actuellement effectuées par l'analyse de l'ADN (Acide DésoxyriboNucléique) au moyen de l'examen du polymorphisme VNTR (Variable Number of Tandem Repeats) qui est un polymorphisme de restriction (RFLP = Restriction Fragments Length Polymorphism). Un VNTR est un polymorphisme de répétition ou de longueur. Il s'agit d'une séquence répétée qui constitue un allèle, dont le nombre de répétitions varie d'un individu à l'autre, et qui se transmet selon les lois de Mendel. Chez un hétérozygote les deux allèles sont visibles; il s'agit donc de marqueurs génétiques co-dominants.  2, record 10, French, - nombre%20variable%20de%20r%C3%A9p%C3%A9titions%20en%20tandem

Record 10,  Textual support,  Spanish

Record number: 10, Textual support number: 1 DEF

Secuencias repetidas de ADN, organizadas en tándem, cuyo número de copias es muy variable según el genotipo.  3, record 10, Spanish, - repeticiones%20en%20t%C3%A1ndem%20de%20n%C3%BAmero%20variable

Record 11,  Textual support,  English

Record number: 11, Textual support number: 1 CONT

In a process called "molecular combing", DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage Lambda DNA molecule to 21.5+/-0.5 micrometers (unextended length, 16.2 micrometers).  1, record 11, English, - molecular%20combing

Record number: 11, Textual support number: 1 OBS

With the combing process, it was possible to :(i) extend a chromosomal Escherichia coli DNA fragment(10^6 base pairs) and(ii) detect a minute quantity of DNA(10^3 molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.  1, record 11, English, - molecular%20combing

Record 11,  Textual support,  French

Record number: 11, Textual support number: 1 CONT

Le peignage moléculaire de l'ADN, découvert en 1994, repose sur deux principes simples : l'ancrage spécifique, dans des conditions physico-chimiques précises, de molécules individuelles d'ADN par leurs extrémités sur des surfaces traitées ou non; la force de traction développée par un ménisque en mouvement; c'est-à-dire la force exercée sur tout objet placée sur la ligne de contact d'une solution avec une surface, lors du déplacement de cette ligne.  3, record 11, French, - peignage%20mol%C3%A9culaire

Record number: 11, Textual support number: 2 CONT

[...] la technique de peignage moléculaire, destinée à étaler des macromolécules (notamment l'ADN), parallèlement les unes aux autres, en les ancrant spécifiquement par leurs extrémités [...].  4, record 11, French, - peignage%20mol%C3%A9culaire

Record number: 11, Textual support number: 1 OBS

Les applications du peignage moléculaire sont la cartographie génétique, le diagnostic génétique et la PCR in situ.  5, record 11, French, - peignage%20mol%C3%A9culaire

Record 11,  Textual support,  Spanish

Record 12,  Textual support,  English

Record number: 12, Textual support number: 1 CONT

[Plasmids] generally account for only a small fraction of the total host cell DNA, they often carry vital genes, such as those that confer resistance to antibiotics. Because it is so much smaller, plasmid DNA can easily be separated from the DNA of the host cell and purified. For use as cloning vectors, such purified plasmid DNA molecules are cut once with a restriction nuclease and then annealed to the DNA fragment that is to be cloned. The hybrid plasmid DNA molecules produced are then reintroduced into bacteria that have been made transiently permeable to macromolecules. Only some of the treated cells will take up a plasmid. They can be selected by the antibiotic resistance conferred on them by the plasmid since they alone will grow in the presence of antibiotic. As these bacteria divide, the plasmid also replicates to produce an enormous number of copies of the original DNA fragment.  1, record 12, English, - plasmid%20DNA

Record 12,  Textual support,  French

Record number: 12, Textual support number: 1 CONT

La parenté entre les ADN de deux plasmides peut être mesurée par hybridation; il peut s'agir d'hybridation entre plasmides entiers ou entre fragments d'ADN plasmidique après coupure par une endonucléase de restriction. L'ADN non radioactif du premier plasmide est fixé soit entier, soit après fragmentation et séparation par électrophorèse, sur un filtre, et l'hybridation se fait avec l'ADN radioactif préparé avec l'autre plasmide. La mesure de la radioactivité fixée sur le filtre se fait soit par comptage de la radioactivité dans le cas des plasmides entiers, soit par autoradiographie pour les ADN fragmentés. Les plasmides appartenant au même groupe d'incompatibilité ont un très fort pourcentage d'homologie ADN-ADN.  2, record 12, French, - ADN%20plasmidique

Record 12,  Textual support,  Spanish

Record 13,  Textual support,  English

Record number: 13, Textual support number: 1 CONT

The amplified fragment length polymorphism(AFLP) technique is one of a number of DNA fingerprinting procedures that takes advantage of the polymerase chain reaction(PCR) to amplify a limited set of DNA fragments from a specific DNA sample...  2, record 13, English, - amplified%20fragment%20length%20polymorphism

Record 13,  Textual support,  French

Record 13,  Textual support,  Spanish

Record 14,  Textual support,  English

Record number: 14, Textual support number: 1 CONT

Fluorescence-based amplified fragment length polymorphism(fbAFLP) is a novel assay based on the fluorescent analysis of an amplified subset of restriction fragments. The fbAFLP assay involves the selective PCR [polymerase chain reaction] amplification of restriction fragments from a total digest of genomic DNA. The ligation of adapters with primer-specific sites coupled with primers containing selective nucleotides allowed the full potential of PCR to be realized while maintaining the advantages of restriction endonuclease analysis. Fluorescence-based fragment analysis with polyacrylamide gel electrophoresis provides the accurate band sizing required for homology assessment.  5, record 14, English, - fluorescent%20amplified%20fragment%20length%20polymorphism

Record 14,  Textual support,  French

Record number: 14, Textual support number: 1 OBS

L'utilisation du sigle anglais dans les textes français est fréquente. Toutefois, pour le moment, il ne semble y avoir aucun terme au long consacré en français.  2, record 14, French, - polymorphisme%20par%20fluorescence%20de%20longueur%20de%20fragments%20amplifi%C3%A9s

Record 14,  Textual support,  Spanish

Record 15,  Textual support,  English

Record number: 15, Textual support number: 1 DEF

Cloning vehicle designed to promote the expression of gene inserts.  2, record 15, English, - expression%20vector

Record number: 15, Textual support number: 1 OBS

Typically, a restriction fragment carrying the regulatory sequences of a gene is ligated to a plasmid containing a restriction fragment possessing the gene but lacking its regulatory sequences. The plasmid with this new combination of DNA sequences is then cloned under circumstances that promote the expression of the gene under the control of the regulatory sequences.  2, record 15, English, - expression%20vector

Record 15,  Textual support,  French

Record number: 15, Textual support number: 1 DEF

Vecteur de clonage portant une cassette qui permet d'obtenir une expression forte et stable d'une gène cloné à son niveau.  2, record 15, French, - vecteur%20d%27expression

Record 15,  Textual support,  Spanish

Record 16,  Textual support,  English

Record number: 16, Textual support number: 1 CONT

The 603 corn line was created through direct DNA transformation by microparticle bombardment of plant cells with DNA-coated gold particles and regeneration of plants by tissue culture on selective medium. The plasmid vector used in the transformation, a gel-isolated linear restriction fragment designated PV-ZMGT32L, contains two adjacent plant gene expression cassettes, each containing a single copy of the CP4 EPSPS encoding gene.  2, record 16, English, - plasmid%20vector

Record 16,  Textual support,  French

Record number: 16, Textual support number: 1 DEF

Vecteur de clonage élaboré à partir d'un plasmide.  2, record 16, French, - vecteur%20du%20plasmide

Record 16,  Textual support,  Spanish

Record 17,  Textual support,  English

Record number: 17, Textual support number: 1 DEF

A cloning vehicle which has a single cleavage site for one or more restriction endonucleases, thus allowing the insertion of a fragment of foreign DNA.  2, record 17, English, - insertion%20vector

Record number: 17, Textual support number: 1 CONT

Avipoxviruses, including Fowlpoxvirus (FPV) have been shown previously to infect mammalian cells but are unable to complete their replication cycle. Avipoxvirus based rabies and HIV vaccines have shown promise in preclinical studies. We have begun work to develop the FPV system for application to HPV CTL. We first constructed a new FPV insertion vector (Fig. 3) which facilitates production of recombinant viruses by co-insertion of the gpt marker gene under the control of a vaccinia virus 19K promoter. The gene of interest is inserted into a polylinker downstream of the vaccinia virus 7.5K promoter.  3, record 17, English, - insertion%20vector

Record 17,  Textual support,  French

Record number: 17, Textual support number: 1 DEF

Vecteur de clonage ne comportant qu'un seul site de coupure pour une enzyme de restriction donnée, permettant ainsi l'insertion d'un fragment d'ADN étranger.  2, record 17, French, - vecteur%20d%27insertion

Record 17,  Textual support,  Spanish

Record 18,  Textual support,  English

Record number: 18, Textual support number: 1 CONT

In all cases, completely digested DNA was well detected from partially digested DNA in real-time measurement. One possible application of this technique would be the detection of changes of restriction enzyme length polymorphism(RFLP) when point mutations occur in the recognition sequence for the enzyme, and the enzyme produces different number of fragment from original sequence. RFLP has been applied from multilocus typing technique of human genom to any organism.  3, record 18, English, - multilocus%20typing%20technique

Record 18,  Textual support,  French

Record number: 18, Textual support number: 1 CONT

Si le typage de souche a souvent conduit à la conclusion que beaucoup d'agents pathogènes se caractérisent par une population à structure clonale, il est important d'établir des relations de liaison génétique. Celles-ci permettent, en effet, de déterminer avec certitude si un génotype, retrouvé chez tous les individus d'une population, peut-être expliqué (ou non) par une association au hasard, par recombinaison, à chaque locus des allèles les plus communs. Les génotypes multilocus obtenus par PCR aident à résoudre ces problèmes et à différencier entre elles les diverses structures de populations.  1, record 18, French, - typage%20g%C3%A9nomique%20multilocus

Record number: 18, Textual support number: 2 CONT

L'application la plus puissante de la PCR visera les étu des entre espèces via le typage de souches et le typage génomique multilocus.  1, record 18, French, - typage%20g%C3%A9nomique%20multilocus

Record number: 18, Textual support number: 1 OBS

LE TECHNOSCOPE  2, record 18, French, - typage%20g%C3%A9nomique%20multilocus

Record 18,  Textual support,  Spanish

Record 19,  Textual support,  English

Record number: 19, Textual support number: 1 CONT

Primed in situ labeling(PRINS) is a fast alternative to traditional in situ hybridization, requiring only a tew handling steps(1, 2). In the PRINS reaction, an unlabeled sequence-specific oligonucleotide primer or short DNA fragment is hybridized(annealed) to denatures chromosomal DNA and extended in situ in the presence of hapten-labeled nucleotide triphos phates by a suitable DNA polymerase(3). The technique is especially suited for the rapid identification of chromo somes with oligonucleotide primers specific for individual chromosomal sequences and chromosomal elements like centromer or satellite sequences.  2, record 19, English, - primed%20in%20situ%20labeling%20of%20nucleic%20acids

Record 19,  Textual support,  French

Record number: 19, Textual support number: 1 CONT

La technique PRINS (primed in situ DNA synthesis), encore dénommée amorçage in situ, permet de localiser au niveau chromosomique des séquences répétées et présentes en de nombreuses copies. Elle nécessite une amorce nucléotidique capable de s'hybrider à la séquence d'ADN dénaturé. L'hybridation de l'amorce est suivie d'une extension in situ par incorporation, en présence de la Taq polymérase [...]  1, record 19, French, - technique%20PRINS

Record 19,  Textual support,  Spanish

Record 20,  Textual support,  English

Record number: 20, Textual support number: 1 CONT

The case of insulin demonstrates another possibility in that here the donor DNA was a chemically synthesized gene fragment.  1, record 20, English, - chemically%20synthesized

Record 20,  Textual support,  French

Record number: 20, Textual support number: 1 CONT

Le cas de l'insuline met en évidence une autre possibilité en ce sens que l'ADN donneur est dans ce cas un fragment de gène synthétisé par voie chimique.  1, record 20, French, - synth%C3%A9tis%C3%A9%20par%20voie%20chimique

Record 20,  Textual support,  Spanish