TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 CONT

Gene trapping in murine embryonic stem cells is a proven method for the simultaneous identification and mutation of genes in the mouse. Gene trap vectors are designed to detect insertions within genes through the production of a fusion mRNA transcript, making the identification of the endogenous gene possible by 5’ rapid amplification of cDNA ends(RACE).  1, record 1, English, - gene%20trapping

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 CONT

La recherche de gènes essentiels au développement embryonnaire de la souris a été abordée par la mise en œuvre de stratégies variées. Comparée à toutes ces approches, la stratégie de piégeage de gènes présente un important avantage : elle associe l'isolement et la caractérisation moléculaire d'un gène à l'étude de son profil d'expression ainsi qu'à la détermination de sa fonction in vivo.  1, record 1, French, - pi%C3%A9geage%20de%20g%C3%A8nes

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

In contrasts to protoplast fusion which is particularly useful for combining large parts of the genome in a mixed cytoplasm, especially where the characteristics of interest are controlled in a complex manner by a large number of genes, the power of recombinant DNA technology is greatest when small numbers of individual genes controlling known gene products are involved.  1, record 2, English, - mixed%20cytoplasm

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

Contrairement à la fusion de protoplastes qui est particulièrement utile pour combiner de grandes parties d'un génome dans un cytoplasme mixte, surtout lorsque les caractéristiques qui présentent de l'intérêt sont contrôlées de manière complexe par un grand nombre de gènes, la technologie de l'ADN recombinant offre les possibilités les plus grandes lorsque l'on met en œuvre un petit nombre de gènes bien déterminés qui régissent des produits connus.  1, record 2, French, - cytoplasme%20mixte

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

A chromosome abnormality caused by rearrangement of parts between nonhomologous chromosomes.  2, record 3, English, - chromosome%20translocation

Record number: 3, Textual support number: 1 OBS

A gene fusion may be created when the translocation joins two otherwise separated genes, the occurrence of which is common in cancer.  2, record 3, English, - chromosome%20translocation

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

Mécanisme par lequel deux chromosomes non homologues échangent une partie de leur matériel génétique.  3, record 3, French, - translocation%20chromosomique

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 DEF

Distinct polypeptide chain in which lies the domain accommodating the catalytic site of complex enzymes. It is completely separated from the domain building up the binding site for the regulatory effector(s). From an evolutionary viewpoint, it is probable that gene fusion did occur in most cases rather than that originally continuous polypeptide chains have separated. In some oligomeric enzymes the allosteric site may have appeared as a result of gene duplication, e. g. in glutamate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase, whereas in others by acquisition of a foreign structural gene, e. g. the NAD-binding domain in glycogen phosphorylase. However, in present day complex enzymes the catalytic and regulatory protein units remained separate, but associable, entities.  2, record 4, English, - catalytic%20subunit

Record number: 4, Textual support number: 1 CONT

The ATCase of E. coli has ... a molecular weight of about 310,000 but can be dissociated by treatment with mercurial reagents to yield two identical catalytic subunits and three identical regulatory subunits. Each of the two catalytic subunits has a molecular weight of about 100,000 and contains three polypeptide chains of molecular weight 34,000 called C chains. Each catalytic subunit contains three binding sites for the substrate aspartate, one on each of the three C chains. The catalytic subunits have enzymatic activity but are not sensitive to the modulator CTP.  3, record 4, English, - catalytic%20subunit

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Lorsque la molécule native d'ATCase est dissociée en un mélange de ses sous-unités catalytiques et régulatrices, ce mélange fait toujours montre d'activité catalytique, mais la réaction n'est pas inhibée par le CTP tant que les sous-unités catalytiques et régulatrices sont séparées les unes des autres. Pour que l'inhibition par le CTP puisse avoir lieu, il faut que les sous-unités catalytiques et régulatrices soient physiquement associées dans la molécule oligomérique d'ATCase intacte.  1, record 4, French, - sous%2Dunit%C3%A9%20catalytique

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 CONT

Conversely, a mother cell-specific pattern of fluorescence was observed in sporangia bearing a transcriptional fusion of gfp to a spore coat protein gene(cotE) under the control of sigma E and an in-frame fusion to a regulatory gene(gerE) under the control of sigma K.  1, record 5, English, - transcriptional%20fusion

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Fusion de gènes intervenant dans la région transcrite mais non traduite.  1, record 5, French, - fusion%20transcriptionnelle

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 CONT

Gene trapping in murine embryonic stem cells is a proven method for the simultaneous identification and mutation of genes in the mouse. Gene trap vectors are designed to detect insertions within genes through the production of a fusion mRNA transcript, making the identification of the endogenous gene possible by 5’ rapid amplification of cDNA ends(RACE). Although the amplification of specific cDNAs can be achieved rapidly, cloning and screening of informative-sized cDNAs has proven to be time consuming.  1, record 6, English, - gene%20trap%20vector

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 CONT

De façon générale, tous les vecteurs de piégeage possèdent d'une part un gène rapporteur qui d'ordinaire est le gène bactérien lacZ (son activité est facilement décelable et n'est pas affectée par de larges fusions à l'extrémité amino-terminale ) et d'autre part, un gène codant pour une protéine de résistance à un facteur de sélection, généralement la néomycine phospho-transférase.  1, record 6, French, - vecteur%20de%20pi%C3%A9geage

Record 6,  Textual support,  Spanish

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 CONT

Virosome Vectors for gene therapy. The long term goal of this work is to develop a targeted, efficient, liposome-based vector for the delivery and insertion of the CFTR gene to lung cells. To do this, a combination of liposometechnology and molecular biology will be used. Three observations have led us to propose the outlined research :(i) The efficiency of liposome fusion to cells or organelles can be greatly enhanced by incorporating viral fusion proteins intovesicles.(ii) After liposome-cell fusion, the contents of the liposomes are released into the cell' s cytoplasm.(iii) By delivering DNA to nonlysosomal compartments of eukarvotic cells the efficiency of transfection can be greatly enhanced...  1, record 7, English, - virosome

Record number: 7, Textual support number: 1 OBS

Excerpt taken from Microsoft Internet Explorer.  1, record 7, English, - virosome

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 DEF

Liposome dans lequel des particules virales ont été introduites.  1, record 7, French, - virosome

Record 7,  Textual support,  Spanish