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FUSION PROTEIN [5 records]

Record 1 2018-02-21

English

Subject field(s)
  • Muscles and Tendons
  • Molecular Biology
DEF

A metalloproteinase protein mediating the fusion of myoblasts into myotubes.

French

Domaine(s)
  • Muscles et tendons
  • Biologie moléculaire
CONT

La fusion dépend également de certaines métalloprotéinases, les meltrines. C'est la recherche de protéines qui joueraient, dans les myoblastes, le même rôle que la fertiline dans la fusion de l'œuf et du spermatozoïde, qui a conduit à l'identification des meltrines.

Spanish

Save record 1

Record 2 2017-12-15

English

Subject field(s)
  • Scientific Research Methods
CONT

[The] method, called bioluminescence resonance energy transfer(BRET), uses a bioluminescent luciferase that is genetically fused to one candidate protein, and a green fluorescent protein mutant fused to another protein of interest. Interactions between the two fusion proteins can bring the luciferase and green fluorescent protein close enough for resonance energy transfer to occur, thus changing the color of the bioluminescent emission.

French

Domaine(s)
  • Méthodes de recherche scientifique
CONT

[...] les techniques de transfert d’énergie de résonance de bioluminescence (BRET), pour étudier la dynamique de signalisation en temps réel dans des cellules vivantes.

Spanish

Save record 2

Record 3 2011-11-02

English

Subject field(s)
  • Bioengineering
  • Molecular Biology
CONT

A far-Western blot(also known as an overlay assay) is used to detect the interaction of a recombinant GST [glutathione S-transferase] fusion protein(produced and purified from bacteria) with a target protein on a membrane.

French

Domaine(s)
  • Technique biologique
  • Biologie moléculaire
CONT

L'utilisation de la technique de Far-Western, dans le but d'identifier les interactions protéine-protéine entre un virus et son vecteur, ont permis d'isoler deux protéines de thrips de 50 et 94 kDa [kiloDaltons] présentant des affinités particulières pour les glycoprotéines du TSWV [tomato spotted wilt virus].

Spanish

Save record 3

Record 4 2011-01-05

English

Subject field(s)
  • Biological Sciences
DEF

Distinct polypeptide chain in which lies the domain accommodating the catalytic site of complex enzymes. It is completely separated from the domain building up the binding site for the regulatory effector(s). From an evolutionary viewpoint, it is probable that gene fusion did occur in most cases rather than that originally continuous polypeptide chains have separated. In some oligomeric enzymes the allosteric site may have appeared as a result of gene duplication, e. g. in glutamate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase, whereas in others by acquisition of a foreign structural gene, e. g. the NAD-binding domain in glycogen phosphorylase. However, in present day complex enzymes the catalytic and regulatory protein units remained separate, but associable, entities.

CONT

The ATCase of E. coli has ... a molecular weight of about 310,000 but can be dissociated by treatment with mercurial reagents to yield two identical catalytic subunits and three identical regulatory subunits. Each of the two catalytic subunits has a molecular weight of about 100,000 and contains three polypeptide chains of molecular weight 34,000 called C chains. Each catalytic subunit contains three binding sites for the substrate aspartate, one on each of the three C chains. The catalytic subunits have enzymatic activity but are not sensitive to the modulator CTP.

French

Domaine(s)
  • Sciences biologiques
CONT

Lorsque la molécule native d'ATCase est dissociée en un mélange de ses sous-unités catalytiques et régulatrices, ce mélange fait toujours montre d'activité catalytique, mais la réaction n'est pas inhibée par le CTP tant que les sous-unités catalytiques et régulatrices sont séparées les unes des autres. Pour que l'inhibition par le CTP puisse avoir lieu, il faut que les sous-unités catalytiques et régulatrices soient physiquement associées dans la molécule oligomérique d'ATCase intacte.

Spanish

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Record 5 2010-04-20

English

Subject field(s)
  • Genetics
CONT

Conversely, a mother cell-specific pattern of fluorescence was observed in sporangia bearing a transcriptional fusion of gfp to a spore coat protein gene(cotE) under the control of sigma E and an in-frame fusion to a regulatory gene(gerE) under the control of sigma K.

French

Domaine(s)
  • Génétique
DEF

Fusion de gènes intervenant dans la région transcrite mais non traduite.

Spanish

Save record 5

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