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GEL FILTRATION CHROMATOGRAPHY [9 records]

Record 1 2016-11-16

English

Subject field(s)
  • Chemical Engineering
DEF

The process by means of which macromolecular species differing in some characteristic (chemical composition, relative molecular mass, branching, stereoregularity, etc.) are separated from each other.

OBS

In general, the separation or isolation of a mixture or a micromolecular complex... Macromolecular components(proteins and other high polymers) can be separated by a number of methods, including electrophoresis, gel filtration, chromatography, centrifugation, foam fractionation, and partition.

OBS

fractionation: term and definition standardized by ISO.

French

Domaine(s)
  • Génie chimique
DEF

Processus par lequel des espèces macromoléculaires différenciées les unes des autres par une caractéristique quelconque (composition chimique, masse moléculaire relative, ramification, stéréorégularité, etc.) sont séparées les unes des autres.

OBS

fractionnement : terme et définition normalisés par l'ISO.

Spanish

Campo(s) temático(s)
  • Ingenieria química
DEF

Proceso por medio del cual especies macromoleculares que difieren en alguna característica (composición química, masa molecular relativa, ramificación, estereoregularidad, etc.) se separan unas de otras.

Save record 1

Record 2 2011-06-23

English

Subject field(s)
  • Analytical Chemistry
  • Biological Sciences
  • Biotechnology
DEF

Chromatographic analysis in which the stationary phase consists of beads of porous polymeric material sold under the trade name Sephadex; the mobile phase is liquid.

CONT

Gel Filtration Chromatography. The gel filtration method is a relatively new fractionation procedure in liquid chromatography for the separation of molecules according to the differences in size and shape and can be considered a a kind of "molecular sieve. "This method has also been referred to in some publications as exclusion chromatography, restricted diffusion chromatography, restricted diffusion chromatography, gel chromatography and gel permeation chromatography, just to mention a few.

OBS

Gel permeation chromatography... is the preferred term for what has also been known variously as exclusion chromatography, gel chromatography and gel filtration.

OBS

"Gel chromatography" is an inclusive term to designate all forms of exclusion chromatography.

Key term(s)
  • SEC

French

Domaine(s)
  • Chimie analytique
  • Sciences biologiques
  • Biotechnologie
DEF

Analyse chromatographique dans laquelle la phase stationnaire est formée de perles de substance poreuse et la phase mobile est liquide.

OBS

La substance poreuse utilisée est connue commercialement sous l'appellation Sephadex.

Key term(s)
  • chromatographie de filtration de gel

Spanish

Campo(s) temático(s)
  • Química analítica
  • Ciencias biológicas
  • Biotecnología
DEF

Procedimiento cromatográfico que separa distintas sustancias en función de sus tamaños moleculares.

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Record 3 2011-06-06

English

Subject field(s)
  • Chemical Elements and Compounds
  • Food Additives
  • Pharmacy
DEF

Any of numerous polysaccharides (C6H10O5)n that yield only glucose on hydrolysis [and are] of very high molecular weight usually obtained by fermentation of sugar with bacteria of the genus Leuconostoc (as L. mesenteroides).

OBS

Used in the brewing industry (antifoaming agent) and in candy. A barley malt substitute.

OBS

It is also used in the production of media for gel filtration chromatography(Sephadex), plasma volume expanders, ion exchangers and flow improvers. It also causes fouling problems and distorts crystal shape, producing long needles, in sugar processing. Dextran is produced commercially by growing Leuconostoc mesenteroides on sucrose-based media.

OBS

Clinical dextran has been standardized to a low molecular weight (75,000).

French

Domaine(s)
  • Éléments et composés chimiques
  • Additifs alimentaires
  • Pharmacie
DEF

Polysaccharide de haut poids moléculaire, produit par la fermentation du saccharose sous l'action de certaines bactéries (Leuconostoc, Acetobacter, etc.). Employé en brasserie (antimousse) et en confiserie. Substitut du malt d'orge.

OBS

Certains dextranes servent en confiserie.

OBS

Le dextrane de synthèse (dextran) est utilisé dans les transfusions sanguines. Les propriétés physico-chimiques de ce produit s'apparentent au plasma.

Key term(s)
  • dextran

Spanish

Campo(s) temático(s)
  • Elementos y compuestos químicos
  • Aditivos alimentarios
  • Farmacia
DEF

Cualquiera de los polisacáridos obtenidos por polimerización de isomaltosa mediante enlaces 1->6.

Save record 3

Record 4 2010-09-15

English

Subject field(s)
  • Biological Sciences
DEF

A form of calcium phosphate used in chromatography, it also contains carbonate, magnesium, citrate, and fluoride ions. It is found in the matrix of bone and the teeth, which gives rigidity to these structures.

CONT

Hydroxyapatite(HA) has been used in batch techniques for enzyme purification over a considerable period.... Bernardi considered that chromatography on HA should be used as an additional tool in the purification of proteins because the basis of separation is different from that of either ion exchange or gel filtration.

French

Domaine(s)
  • Sciences biologiques
DEF

Hydrophosphate de calcium à structure microcristalline, formant le cristal osseux.

CONT

Les protéines peuvent être adsorbées, puis sélectivement éluées sur des colonnes d'un matériel finement divisé relativement inerte qui possède une grande surface par rapport à la taille des particules. (...) Le plus largement et le plus efficacement utilisé de ces adsorbants pour la purification des protéines est l'hydroxyapatite. (...) Vraisemblablement, les groupements chargés négativement des molécules protéiques se lient aux ions Ca2+ dans les cristaux d'hydroxyapatite. Les protéines peuvent être éluées à partir des colonnes d'hydroxyapatite avec des tampons phosphate.

Spanish

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Record 5 2000-10-19

English

Subject field(s)
  • Genetics
DEF

A protein, polypeptide, nucleic acid, oligonucleotide, etc. used as a reference material in analytical techniques such as gel filtration chromatography, electrophoresis and density gradient centrifugation.

French

Domaine(s)
  • Génétique
DEF

Macromolécule (protéine, acide nucléique, etc.), de masse moléculaire connue, utilisée comme référence dans les techniques d'analyse tels la chromatographie, l'électrophorèse et la centrifugation.

Spanish

Save record 5

Record 6 2000-10-19

English

Subject field(s)
  • Biochemistry
DEF

Type of high performance liquid chromatography that uses a gel filtration column.

French

Domaine(s)
  • Biochimie

Spanish

Save record 6

Record 7 1999-08-27

English

Subject field(s)
  • Biochemistry
DEF

Proprietary form of agarose supplied in bead form for use in gel filtration and other types of liquid chromatography.

French

Domaine(s)
  • Biochimie
OBS

Marque déposée d'un gel de dextran modifié utilisé pour effectuer la chromatographie d'exclusion.

Spanish

Save record 7

Record 8 1998-08-28

English

Subject field(s)
  • Genetics
CONT

Immunoglobulin stimulating factor(IPSF) 11f was purified from Namalwa cell lysate by ammonium sulfate precipitation, hydrophobic interaction chromatography and gel filtration.

French

Domaine(s)
  • Génétique

Spanish

Save record 8

Record 9 1991-01-31

English

Subject field(s)
  • Bioengineering
CONT

Implantation of porcine bone morphogenetic protein(pBMP) in the muscle induces differentiation of mesenchymal-type cells and results in endochondral bone formation, pBMP was isolated from porcine demineralized bone matrix and purified by hydroxyapatite chromatography, Sephadex G75 gel filtration, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), preparative isoelectric focusing(IEF), and chromatofocusing fast protein liquid chromatography(FPLC).

OBS

Term and context extracted from the PASCAL data base.

French

Domaine(s)
  • Technique biologique
OBS

Terme tiré de la liste française des descripteurs de l'article (base de données PASCAL).

Spanish

Save record 9

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