TERMIUM Plus^®

Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

Gel produced by cross-linking selected dextran fractions with epichlorhydrin and marketed in bead form. Various types of gels are available, differing in pore size and swelling characteristics, according to the degree of cross-linking.  2, record 1, English, - Sephadex%C2%AE

Record number: 1, Textual support number: 1 CONT

Sephadex is used in gel filtration. Gels in which the matrix is a minor component are used for fractionation of high molecular weight substances; denser gels are used for separation of low molecular weight compounds. Sephadex is available in eight porosities which provide the fractionation of globular proteins with molecular weights of up to 8 x 10⁵ and linear polymers of up to 2 x 10⁵.  2, record 1, English, - Sephadex%C2%AE

Record number: 1, Textual support number: 1 OBS

Sephadex® is a registered trademark of Pharmacia Biotechnology AB.  3, record 1, English, - Sephadex%C2%AE

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Nom déposé d'un gel de dextran modifié, obtenu par réticulation des macromolécules par l'épichlorhydrine pour former un réseau tridimensionnel de chaînes de polysaccharides se présentant sous forme de très petits grains sphériques (perles).  3, record 1, French, - S%C3%A9phadex

Record number: 1, Textual support number: 1 CONT

Les gels de dextrane utilisés (type Sephadex) sont des polyosides comportant un nombre variable de liaisons croisées lesquelles déterminent un degré de porosité; la pénétration des macromolécules dans ces gels est plus ou moins facile selon leur taille d'où le nom de «tamis moléculaires» que l'on donne parfois à ces colonnes de gel de dextrane : les plus grosses molécules, exclues du gel, sortiront les premières de la colonne, tandis que les plus petites, pouvant y pénétrer, seront retardées et sortiront en dernier.  4, record 1, French, - S%C3%A9phadex

Record number: 1, Textual support number: 1 OBS

Sephadex® : marque déposée.  3, record 1, French, - S%C3%A9phadex

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 DEF

Enzyme immobilization method which involves the formation of a highly crosslinked network of a polymer in the presence of an enzyme. Enzyme molecules are physically entrapped within the polymer lattice and cannot permeate out of the gel matrix, but appropriately sized substrate and product molecules can transfer across and within this network to insure a continuous transformation.  2, record 2, English, - lattice%20entrapment

Record number: 2, Textual support number: 1 CONT

Advantages of the lattice entrapment method for immobilizing an enzyme include its overall experimental simplicity, the need for only small amounts of an enzyme in order to produce a water-insoluble enzyme conjugate and the fact that it is a physical method.... Disadvantages of the method also exist.... The exact physical nature of the crosslinked polymers is often quite important for obtaining high activity.... A considerable disadvantage of this method is leakage of an enzyme from within the crosslinked polymeric network.  1, record 2, English, - lattice%20entrapment

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 DEF

Une des techniques d'immobilisation par inclusion dans laquelle l'enzyme est solubilisée ou dispersée dans une solution d'un monomère qui est ensuite polymérisé en présence d'un agent de réticulation et d'un colloïde protecteur, albumine, agarose ou dextrane.  2, record 2, French, - inclusion%20dans%20une%20matrice

Record number: 2, Textual support number: 1 CONT

Grâce à leur spécificité et leur rapidité d'action dans des conditions douces, les enzymes peuvent constituer des réactifs très précieux offrant d'énormes possibilités. Cependant, leur emploi en solution reste limité en raison de leur fragilité (dénaturation) et de la difficulté de récupération de l'enzyme pour une utilisation répétée. Ces inconvénients ont été considérablement réduits par l'immobilisation des enzymes, qui a pu être réalisée par des procédés très divers : réticulation de la protéine enzymatique, conduisant à des agrégats insolubles, inclusion dans les mailles d'un gel (entrappement) ou en microcapsules poreuses, ou fixation sur un support solide insoluble (matrice) par adsorption, ou mieux par liaison covalente.  3, record 2, French, - inclusion%20dans%20une%20matrice

Record number: 2, Textual support number: 1 OBS

Le terme "entrappement" est quelquefois utilisé dans les ouvrages français. Emploi à déconseiller.  2, record 2, French, - inclusion%20dans%20une%20matrice

Record 2,  Textual support,  Spanish

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 DEF

[A] separation based mainly upon exclusion effects, such as differences in molecular size and/or shape ... or in charge ...  1, record 3, English, - permeation%20chromatography

Record number: 3, Textual support number: 2 DEF

A form of liquid chromatography in which the separation of the sample components is based on the differences in the size and/or shape of the molecules or in the charge ...  1, record 3, English, - permeation%20chromatography

Record number: 3, Textual support number: 1 OBS

"Gel chromatography", in which the stationary phase is either a swollen gel or a rigid three-dimensional silica or polymetric matrix, is the most widely used permeation technique.  2, record 3, English, - permeation%20chromatography

Record 3,  Textual support,  French

Record number: 3, Textual support number: 1 DEF

[...] séparation [...] basée principalement sur les effets d'exclusion liés à la différence de taille et/ou de formes de molécules à séparer [...] ou de charge ionique [...]  1, record 3, French, - chromatographie%20par%20perm%C3%A9ation

Record 3,  Textual support,  Spanish

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 DEF

A matrix consisting of one or several active agents mixed with a gel compound that releases the active agents slowly as the gel becomes hydrated in the gastrointestinal tract.  2, record 4, English, - hydrophilic%20matrix

Record 4,  Textual support,  French

Record number: 4, Textual support number: 1 CONT

Les matrices hydrophiles résultent du mélange d'une ou de plusieurs substances actives avec un agent gélifiant. La libération se déroule en plusieurs étapes. Au contact de l'eau ou des fluides digestifs, une très petite fraction de la substance active se dissout immédiatement. Puis la pénétration progressive du solvant permet l'hydratation du système et la gélification des macromolécules, lesquelles forment une couche visqueuse de plus en plus épaisse à partir de laquelle la substance médicamenteuse est libérée après dissolution et diffusion dans le gel.  1, record 4, French, - matrice%20hydrophile

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 CONT

Three families of repetitive sequences have been studied in most detail, including the 35-40 bp [base pair] repetitive extragenic palindromic(REP) sequence, the 124-127 bp enterobacterial repetitive intergenic consensus(ERIC) sequence, and the 154 bp BOX element. These sequences appear to be located in distinct, intergenic positions all around the chromosome. The repetitive elements may be present in both orientations on the chromosome, and PCR [polymerase chain reaction] primers have been designed to "read outward" from the inverted repeats in REP and ERIC, and from the boxA subunit of BOX. The use of the above primer(s) and PCR leads to the selective amplification of distinct genomic regions located between REP, ERIC or BOX sequences. The corresponding protocols are referred to as REP-PCR, ERIC-PCR and BOX-PCR, respectively, and rep-PCR collectively. Amplified bands are size fractionated through a gel matrix to yield fingerprint patterns resembling "bar codes", analogous to UPC codes used in grocery stores, and function as a signature for specific bacterial strains.  3, record 5, English, - BOX%2DPCR

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Technique d'amplification génique effectuée au moyen d'amorces de type BOX afin d'établir l'empreinte génétique d'un microorganisme.  2, record 5, French, - BOX%2DPCR

Record number: 5, Textual support number: 1 OBS

L'utilisation de l'article semble rare. Par exemple, on parlerait plutôt «d'empreinte génétique par BOX-PCR» que «d'empreinte génétique par la BOX-PCR».  2, record 5, French, - BOX%2DPCR

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 DEF

Analytical or separation technique in which charged compounds are separated under the influence of an electrical field(electrophoresis) with a gel being used as the support matrix.  2, record 6, English, - gel%20electrophoresis

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 DEF

Méthode de séparation des molécules fondée sur leur migration différentielle au travers d'une matrice [...] lorsqu'on applique un potentiel électrique.  3, record 6, French, - %C3%A9lectrophor%C3%A8se%20en%20gel

Record 6,  Textual support,  Spanish

Record 7,  Textual support,  English

Record number: 7, Textual support number: 1 DEF

The pH at which a substance is electrically neutral or at which it is at its minimum ionization.  4, record 7, English, - isoelectric%20point

Record number: 7, Textual support number: 2 DEF

The pH at which a molecule (usually an amino acid of protein) has no net charge and will not move in an electric field.  5, record 7, English, - isoelectric%20point

Record number: 7, Textual support number: 1 OBS

Iso-electric focusing : A technique for separating proteins according to their iso-electric points. The mixture of proteins is placed in a pH gradient established by ampolines in an electric field across a liquid or a gel matrix; the proteins move in the electric field until they reach their iso-electric points in the pH gradient where, having lost net charge, they are focused.  2, record 7, English, - isoelectric%20point

Record 7,  Textual support,  French

Record number: 7, Textual support number: 1 OBS

Le point isoélectrique est à distinguer du point iso-ionique caractérisé par l'égalité du nombre des charges positives et négatives portées par la molécule, bien que ces deux points soient très proches et habituellement confondus.  3, record 7, French, - point%20iso%2D%C3%A9lectrique

Record 7,  Textual support,  Spanish

Record 8,  Textual support,  English

Record number: 8, Textual support number: 1 DEF

An electrode that incorporates an enzyme into its design and that is specific for measuring the concentration of a reactant or a product of the reaction catalyzed by the enzyme. The enzyme is frequently trapped within a gel matrix around the electrode or trapped within a liquid film in a cellophane membrane.  1, record 8, English, - enzyme%20electrode

Record 8,  Textual support,  French

Record number: 8, Textual support number: 1 DEF

Électrode [...] constituée d'une électrode classique recouverte d'un film porteur d'enzymes immobilisées.  2, record 8, French, - %C3%A9lectrode%20%C3%A0%20enzymes

Record number: 8, Textual support number: 1 OBS

Les enzymes transforment la substance à doser en un produit auquel l'électrode est sensible. Ces capteurs dosent en continu le glucose, le lactose, l'urée, certains acides aminés.  2, record 8, French, - %C3%A9lectrode%20%C3%A0%20enzymes

Record 8,  Textual support,  Spanish

Record 9,  Textual support,  English

Record number: 9, Textual support number: 1 CONT

Capillary gel electrophoresis(CGE) is the CE-analog of traditional slab-gel electrophoresis and is used for the size-based separation of biological macromolecules such as olgonucleotides, DNA restriction fragments and proteins. The separation is performed by filling the capillary with a sieve-like matrix, for example, cross-linked polyacrylamide, agarose or even solutions of linear polymer. The main advantages over slab-gel electrophoresis are a wider range of gel matrix and composition, online detection, improved quantification and automation.  1, record 9, English, - capillary%20gel%20electrophoresis

Record 9,  Textual support,  French

Record number: 9, Textual support number: 1 CONT

L'électrophorèse capillaire en gel est fondée sur l'utilisation de capillaires de silice remplis de gel de polyacrylamide ou d'agarose. Cette technique est dotée d'une résolution importante autorisant son application au séquençage. En effet, elle permet de distinguer de courts fragments d'ADN (inférieurs à 1000 bases) dont la taille ne varie que d'une base.  1, record 9, French, - %C3%A9lectrophor%C3%A8se%20capillaire%20en%20gel

Record 9,  Textual support,  Spanish

Record 10,  Textual support,  English

Record number: 10, Textual support number: 1 CONT

Implantation of porcine bone morphogenetic protein(pBMP) in the muscle induces differentiation of mesenchymal-type cells and results in endochondral bone formation, pBMP was isolated from porcine demineralized bone matrix and purified by hydroxyapatite chromatography, Sephadex G75 gel filtration, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), preparative isoelectric focusing(IEF), and chromatofocusing fast protein liquid chromatography(FPLC).  1, record 10, English, - fast%20protein%20liquid%20chromatography

Record number: 10, Textual support number: 1 OBS

Term and context extracted from the PASCAL data base.  1, record 10, English, - fast%20protein%20liquid%20chromatography

Record 10,  Textual support,  French

Record number: 10, Textual support number: 1 OBS

Terme tiré de la liste française des descripteurs de l'article (base de données PASCAL).  1, record 10, French, - chromatographie%20FPLC

Record 10,  Textual support,  Spanish