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GENE AMPLIFICATION [7 records]
Record 1 - internal organization data 2017-01-10
Record 1, English
Record 1, Subject field(s)
- Biotechnology
Record 1, Main entry term, English
- gene trapping
1, record 1, English, gene%20trapping
correct
Record 1, Abbreviations, English
Record 1, Synonyms, English
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
Gene trapping in murine embryonic stem cells is a proven method for the simultaneous identification and mutation of genes in the mouse. Gene trap vectors are designed to detect insertions within genes through the production of a fusion mRNA transcript, making the identification of the endogenous gene possible by 5’ rapid amplification of cDNA ends(RACE). 1, record 1, English, - gene%20trapping
Record 1, French
Record 1, Domaine(s)
- Biotechnologie
Record 1, Main entry term, French
- piégeage de gènes
1, record 1, French, pi%C3%A9geage%20de%20g%C3%A8nes
correct, masculine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- technique de piégeage des gènes 2, record 1, French, technique%20de%20pi%C3%A9geage%20des%20g%C3%A8nes
proposal, feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
La recherche de gènes essentiels au développement embryonnaire de la souris a été abordée par la mise en œuvre de stratégies variées. Comparée à toutes ces approches, la stratégie de piégeage de gènes présente un important avantage : elle associe l'isolement et la caractérisation moléculaire d'un gène à l'étude de son profil d'expression ainsi qu'à la détermination de sa fonction in vivo. 1, record 1, French, - pi%C3%A9geage%20de%20g%C3%A8nes
Record 1, Spanish
Record 1, Textual support, Spanish
Record 2 - internal organization data 2012-05-22
Record 2, English
Record 2, Subject field(s)
- Biochemistry
Record 2, Main entry term, English
- double minute chromosome
1, record 2, English, double%20minute%20chromosome
correct
Record 2, Abbreviations, English
- Dm 2, record 2, English, Dm
correct
Record 2, Synonyms, English
Record 2, Textual support, English
Record number: 2, Textual support number: 1 CONT
Recent experiments have shown that gene amplification can be mediated by submicroscopic, autonomously replicating, circular extrachromosomal molecules. We refer to those molecules as episomes... The experiments... explore the way episomes are formed and their fate in the cell over time. The data reveal that in our system the episomes are initially 250 kilobases, but gradually enlarge until they become double minute chromosomes. In addition, we show that episomes or double minute chromosomes can integrate into chromosomes. 3, record 2, English, - double%20minute%20chromosome
Record 2, French
Record 2, Domaine(s)
- Biochimie
Record 2, Main entry term, French
- chromosome minuscule double
1, record 2, French, chromosome%20minuscule%20double
correct, masculine noun, standardized
Record 2, Abbreviations, French
Record 2, Synonyms, French
- chromosome minute 2, record 2, French, chromosome%20minute
correct, masculine noun
- chromosome double-minutes 2, record 2, French, chromosome%20double%2Dminutes
avoid, masculine noun
Record 2, Textual support, French
Record number: 2, Textual support number: 1 DEF
Fragment d'ADN chromosomique dépourvu de centromère, présent dans les lignées cellulaires instables. [Définition normalisée par l’AFNOR et reproduite avec son autorisation.] 1, record 2, French, - chromosome%20minuscule%20double
Record number: 2, Textual support number: 1 CONT
L'amplification d'ADN peut parfois être visualisée en cytogénétique, sous deux formes différentes. La première, extrachromosomique, correspond aux chromosomes minutes. Structures circulaires super-enroulées, souvent appelées doubles-minutes (Dm) du fait de leur aspect dupliqué, ces éléments se répliquent de manière autonome et, ne possédant pas de centromère, sont distribués inégalement lors de la mitose : en absence de pression sélective, ils sont rapidement perdus dans la population cellulaire. 2, record 2, French, - chromosome%20minuscule%20double
Record number: 2, Textual support number: 1 OBS
chromosome minuscule double : terme normalisé par l'AFNOR. 3, record 2, French, - chromosome%20minuscule%20double
Record 2, Spanish
Record 2, Textual support, Spanish
Record 3 - internal organization data 2010-12-09
Record 3, English
Record 3, Subject field(s)
- Genetics
Record 3, Main entry term, English
- extrachromosomal gene
1, record 3, English, extrachromosomal%20gene
correct
Record 3, Abbreviations, English
Record 3, Synonyms, English
Record 3, Textual support, English
Record number: 3, Textual support number: 1 CONT
Homogeneously staining regions(HSRs) have been recognized as cytogenetic manifestations of intrachromosomal or extrachromosomal gene amplification. Gene amplification has also been associated with both tumor stage and disease progression. 2, record 3, English, - extrachromosomal%20gene
Record 3, French
Record 3, Domaine(s)
- Génétique
Record 3, Main entry term, French
- gène extrachromosomique
1, record 3, French, g%C3%A8ne%20extrachromosomique
correct, masculine noun
Record 3, Abbreviations, French
Record 3, Synonyms, French
Record 3, Textual support, French
Record number: 3, Textual support number: 1 OBS
Terminologie du génie génétique : Vocabulaire bilingue tiré de l'Arrêté du 14 septembre 1990 (JO du 26 septembre 1990), Commission de terminologie pour le vocabulaire des sciences et des techniques de l'agriculture et de l'agro-alimentaire; Avis de la Délégation générale à la langue française et Avis du Conseil international de la langue française. 1, record 3, French, - g%C3%A8ne%20extrachromosomique
Record 3, Spanish
Record 3, Campo(s) temático(s)
- Genética
Record 3, Main entry term, Spanish
- gen extracromosómico
1, record 3, Spanish, gen%20extracromos%C3%B3mico
correct, masculine noun
Record 3, Abbreviations, Spanish
Record 3, Synonyms, Spanish
Record 3, Textual support, Spanish
Record 4 - internal organization data 2005-06-02
Record 4, English
Record 4, Subject field(s)
- Genetics
- Molecular Biology
Record 4, Main entry term, English
- DNA puff
1, record 4, English, DNA%20puff
correct
Record 4, Abbreviations, English
Record 4, Synonyms, English
Record 4, Textual support, English
Record number: 4, Textual support number: 1 DEF
An area on a chromosome where transcription of DNA into mRNA, or replication of DNA is occurring, resulting in an enlarged, puffy appearance of the area. 2, record 4, English, - DNA%20puff
Record number: 4, Textual support number: 1 CONT
To address this question, we are using the DNA puffs of the giant salivary gland chromosomes of the fly Sciara coprophila. DNA puffs represent sites of intrachromosomal gene amplification and are an excellent model for study of DNA replication. 3, record 4, English, - DNA%20puff
Record number: 4, Textual support number: 1 OBS
A DNA puff is similar to a chromosome puff, except it is generalised to any type of chromosome in any type of organism. 4, record 4, English, - DNA%20puff
Record 4, French
Record 4, Domaine(s)
- Génétique
- Biologie moléculaire
Record 4, Main entry term, French
- renflement d'ADN
1, record 4, French, renflement%20d%27ADN
proposal, see observation, masculine noun
Record 4, Abbreviations, French
Record 4, Synonyms, French
- boursouflure d'ADN 2, record 4, French, boursouflure%20d%27ADN
see observation, feminine noun
- boursoufflure d'ADN 3, record 4, French, boursoufflure%20d%27ADN
see observation, feminine noun
Record 4, Textual support, French
Record number: 4, Textual support number: 1 DEF
Bande d'un chromosome où s'effectue la transcription de l'ARN en ARN messager ou la réplication de l'ADN, et qui donne lieu à un renflement de cette zone. 1, record 4, French, - renflement%20d%27ADN
Record number: 4, Textual support number: 1 OBS
renflement d'ADN : Le renflement d'ADN se distingue du renflement chromosomique, car il se produit dans n'importe quel type de chromosomes (et non pas seulement sur les chromosomes polytènes) et pour tout type d'organisme. 1, record 4, French, - renflement%20d%27ADN
Record number: 4, Textual support number: 2 OBS
On écrirait mieux «boursoufflure». 4, record 4, French, - renflement%20d%27ADN
Record number: 4, Textual support number: 3 OBS
boursoufflure : Cette graphie, puisée des Rectifications de l'orthographe recommandées par le Conseil supérieur de la langue française, est attestée dans le Petit Robert (2004). 3, record 4, French, - renflement%20d%27ADN
Record 4, Spanish
Record 4, Textual support, Spanish
Record 5 - internal organization data 1999-03-26
Record 5, English
Record 5, Subject field(s)
- Biochemistry
Record 5, Main entry term, English
- gene trap vector
1, record 5, English, gene%20trap%20vector
correct
Record 5, Abbreviations, English
Record 5, Synonyms, English
Record 5, Textual support, English
Record number: 5, Textual support number: 1 CONT
Gene trapping in murine embryonic stem cells is a proven method for the simultaneous identification and mutation of genes in the mouse. Gene trap vectors are designed to detect insertions within genes through the production of a fusion mRNA transcript, making the identification of the endogenous gene possible by 5’ rapid amplification of cDNA ends(RACE). Although the amplification of specific cDNAs can be achieved rapidly, cloning and screening of informative-sized cDNAs has proven to be time consuming. 1, record 5, English, - gene%20trap%20vector
Record 5, French
Record 5, Domaine(s)
- Biochimie
Record 5, Main entry term, French
- vecteur de piégeage
1, record 5, French, vecteur%20de%20pi%C3%A9geage
correct, masculine noun
Record 5, Abbreviations, French
Record 5, Synonyms, French
Record 5, Textual support, French
Record number: 5, Textual support number: 1 CONT
De façon générale, tous les vecteurs de piégeage possèdent d'une part un gène rapporteur qui d'ordinaire est le gène bactérien lacZ (son activité est facilement décelable et n'est pas affectée par de larges fusions à l'extrémité amino-terminale ) et d'autre part, un gène codant pour une protéine de résistance à un facteur de sélection, généralement la néomycine phospho-transférase. 1, record 5, French, - vecteur%20de%20pi%C3%A9geage
Record 5, Spanish
Record 5, Textual support, Spanish
Record 6 - internal organization data 1994-03-22
Record 6, English
Record 6, Subject field(s)
- Cytology
- Genetics
Record 6, Main entry term, English
- self-splicing intron
1, record 6, English, self%2Dsplicing%20intron
correct
Record 6, Abbreviations, English
Record 6, Synonyms, English
Record 6, Textual support, English
Record number: 6, Textual support number: 1 CONT
[Request for] funding to study the distribution and behaviour of a Group I intron found in the 25S rRNA gene of certain strains of Candida albicans [However] a number of the proposed experimental approaches ... do not take advantage of information that has been accumulating about this class of self-splicing introns over recent years .... 1, record 6, English, - self%2Dsplicing%20intron
Record number: 6, Textual support number: 2 CONT
In vitro genetic analysis of the Tetrahymena self-splicing intron... We have now developed an in vitro selection and amplification system, and used it to analyse the self-splicing Tetrahymena ribozyme... A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii... Sequence requirements for branch formation in a group II self-splicing intron... The nrdB gene of bacteriophage T4 codes for the small subunit of ribonucleotide reductase and contains a 598 base pair self-splicing intron which is closely related to other group I introns of T4 and eukaryotes... Group II and nuclear pre-mRNAs introns share a common splicing pathway involving a lariat intermediate, as well as some primary sequence similarities at the splice junctions. In this work, we analyze the role of the conserved nucleotides at the first and penultimate positions(G1 and A886) of a group II self-splicing intron... [Source : PASCAL database]. 2, record 6, English, - self%2Dsplicing%20intron
Record 6, French
Record 6, Domaine(s)
- Cytologie
- Génétique
Record 6, Main entry term, French
- autoépissage d'intron
1, record 6, French, auto%C3%A9pissage%20d%27intron
masculine noun
Record 6, Abbreviations, French
Record 6, Synonyms, French
Record 6, Textual support, French
Record number: 6, Textual support number: 1 OBS
splicing = épissage : excision des séquences non codantes; autoépissage ou auto-dépistage : se dit du mécanisme d'excision des introns et de raboutage des exons au cours de la maturation des transcrits. 1, record 6, French, - auto%C3%A9pissage%20d%27intron
Record number: 6, Textual support number: 2 OBS
[intron :] Toute séquence transcrite éliminée par épissage au cours de la maturation du transcrit primaire, donc non retrouvée dans le messager cytosolique mûr. Il n'existe aucune règle concernant la longueur et le nombre des introns et des exons. Ceux-ci varient considérablement d'un gène à l'autre. 2, record 6, French, - auto%C3%A9pissage%20d%27intron
Record 6, Key term(s)
- introns impliqués dans le processus/mécanisme d'autoépissage
Record 6, Spanish
Record 6, Textual support, Spanish
Record 7 - internal organization data 1985-03-25
Record 7, English
Record 7, Subject field(s)
- Genetics
Record 7, Main entry term, English
- gene amplification technique 1, record 7, English, gene%20amplification%20technique
Record 7, Abbreviations, English
Record 7, Synonyms, English
Record 7, Textual support, English
Record number: 7, Textual support number: 1 CONT
The technique of gene amplification has been widely exploited in bacteria such as Escherichia coli. 1, record 7, English, - gene%20amplification%20technique
Record 7, French
Record 7, Domaine(s)
- Génétique
Record 7, Main entry term, French
- technique d'amplification des gènes
1, record 7, French, technique%20d%27amplification%20des%20g%C3%A8nes
feminine noun
Record 7, Abbreviations, French
Record 7, Synonyms, French
Record 7, Textual support, French
Record number: 7, Textual support number: 1 CONT
On a surtout utilisé la technique d'amplification des gènes sur des bactéries comme Escherichia coli. 1, record 7, French, - technique%20d%27amplification%20des%20g%C3%A8nes
Record 7, Spanish
Record 7, Textual support, Spanish
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