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Record 1,  Textual support,  English

Record number: 1, Textual support number: 1 DEF

An analytical or separation procedure similar to gel electrophoresis. It is used to separate proteins and other charged molecules on the basis of their isoelectric point. A series of ampholytes are used to establish a pH gradient within a support gel. The charged molecules migrate through the gel until they reach an equilibrium point at which they carry no net charge and thus are not affected by the electric field across the gel.  1, record 1, English, - isoelectric%20focusing

Record 1,  Textual support,  French

Record number: 1, Textual support number: 1 DEF

Méthode de séparation très résolutive des protéines qui repose sur la distinction de ces macromolécules selon leur point isoélectrique (pHi). Chaque protéine en effet, comme n'importe quel ampholyte, est définie par son point isoélectrique, valeur du pH du milieu pour lequel sa charge électrique résultante est nulle. Placée à un pH supérieur à son pHi la protéine se charge négativement par perte de protons. A un pH inférieur au pHi, la protéine se charge positivement par capture de protons. Sous l'influence d'un champ électrique continu, les protéines migrent vers la zone de pH "en équilibre" avec leur pH isoélectrique, c'est-à-dire dans la zone où elles seront électriquement neutres.  2, record 1, French, - %C3%A9lectrofocalisation

Record 1,  Textual support,  Spanish

Record 2,  Textual support,  English

Record number: 2, Textual support number: 1 CONT

Examination of whole cell lipid profiles for PM1 [Methylibium petroleiphilum strain] and YM1 [Bacillus sp. strain] indicates that they are not identical strains. Thermal gradient gel electrophoresis(TGGE) analysis of 16S rRNA gene fragments from the two strains, however, showed no difference between them. 16S rDNA sequence analysis of strains PM1 and YM1 revealed that they are both most closely related to the genus Sphingomonas.  2, record 2, English, - thermal%20gradient%20gel%20electrophoresis

Record 2,  Textual support,  French

Record number: 2, Textual support number: 1 CONT

L'analyse de fragments obtenus après digestion de l'ADN ribosomal amplifié par des enzymes de restriction (ARDRA, amplified ribosomal DNA restriction analysis), la séparation des fragments amplifiés par électrophorèse sur gel avec gradient de dénaturation (DGGE, TGGE: thermal gradient gel electrophoresis) permettent de caractériser les communautés microbiennes par leur signature moléculaire.  3, record 2, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record number: 2, Textual support number: 1 OBS

TGGE est le sigle anglais.  4, record 2, French, - %C3%A9lectrophor%C3%A8se%20sur%20gel%20en%20gradient%20de%20temp%C3%A9rature

Record 2,  Textual support,  Spanish

Record number: 2, Textual support number: 1 DEF

Método para separar fragmentos de ADN según su movilidad bajo condiciones que favorecen progresivamente la desnaturalización por calor.  1, record 2, Spanish, - electroforesis%20en%20gel%20de%20gradiente%20t%C3%A9rmico

Record 3,  Textual support,  English

Record number: 3, Textual support number: 1 CONT

High-resolution HLA-DRB [human leucocyte antigen-5, 6-dichloro-furanosyl-benzimidazole riboside] typing is required for bone marrow transplantation between unrelated donors and recipients and also for identification of novel HLA-DRB alleles. Here we describe a method for the unambiguous identification of HLA-DRB alleles using the polymerase chain reaction(PCR), denaturing gradient gel electrophoresis(DGGE) and direct sequencing.  1, record 3, English, - denaturing%20gradient%20gel%20electrophoresis

Record 3,  Textual support,  French

Record 3,  Textual support,  Spanish

Record number: 3, Textual support number: 1 DEF

Método de electroforesis para separar fragmentos de ADN del mismo tamaño en función de su secuencia.  1, record 3, Spanish, - electroforesis%20en%20gel%20desnaturalizante%20en%20gradiente

Record 4,  Textual support,  English

Record number: 4, Textual support number: 1 CONT

Capillary electrophoresis methodologies will allow the continuous separation and on-line quantification of both high and low molecular weight proteins and polypeptides replacing, updating and improving gradient gel procedures.  1, record 4, English, - gradient%20gel%20procedure

Record 4,  Textual support,  French

Record 4,  Textual support,  Spanish

Record 5,  Textual support,  English

Record number: 5, Textual support number: 1 DEF

A protein, polypeptide, nucleic acid, oligonucleotide, etc. used as a reference material in analytical techniques such as gel filtration chromatography, electrophoresis and density gradient centrifugation.  1, record 5, English, - molecular%20weight%20marker

Record 5,  Textual support,  French

Record number: 5, Textual support number: 1 DEF

Macromolécule (protéine, acide nucléique, etc.), de masse moléculaire connue, utilisée comme référence dans les techniques d'analyse tels la chromatographie, l'électrophorèse et la centrifugation.  2, record 5, French, - %C3%A9talon%20de%20masse%20mol%C3%A9culaire

Record 5,  Textual support,  Spanish

Record 6,  Textual support,  English

Record number: 6, Textual support number: 1 CONT

Complete Electrophoresis System.... Modular system based on the Multiphor II Electrophoresis Unit can adapt to perform high performance electrofocusing, analytical electrofocusing, preparative electrofocusing, preparative isotachophoresis, immuno and agarose electrophoresis, titration curves, gradient gels, SDS gels, polyacrylamide gel electrophoresis(PAGE) and two-dimensional electrophoresis.  1, record 6, English, - electrophoresis%20system

Record 6,  Textual support,  French

Record number: 6, Textual support number: 1 CONT

Système complet d'électrophorèse (...). Le système modulaire fondé sur l'appareil d'électrophorèse Multiphor II peut s'adapter pour effectuer l'électrofocalisation haute performance, l'électrofocalisation analytique, l'électrofocalisation préparative, l'isotachophorèse préparative, [etc].  1, record 6, French, - syst%C3%A8me%20d%27%C3%A9lectrophor%C3%A8se

Record 6,  Textual support,  Spanish