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LAY HOUR [1 record]

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Record 1 1991-06-06

English

Subject field(s)
  • Molecular Biology
  • Biochemistry
CONT

Replica filters are made as follows : A. Prepare in advance a stack of sterile Whatman 3MM filters(one for each filter plus a few spares). B. Peel the master nitrocellulose filter off the storage plate and lay it colony side up on a dampened pad of sterile, 3MM filter paper. C. Number a damp, sterile nitrocellulose filter and lay it on the master nitrocellulose filter. D. Press the two filters firmly together with a velvet replica-plating tool. E. Orient the two filters by placing a series of holes made in the pair of filters with an 18-gauge needle. F. Peel apart the filters. Lay the replica on a fresh agar plate and incubate at 37 ° C until colonies appear. G. Replace the master nitrocellulose filter on a fresh agar plate containing 25% glycerol. Incubate for 1 hour at 37 ° C and then freeze as described in step 6 above.

French

Domaine(s)
  • Biologie moléculaire
  • Biochimie

Spanish

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